Padilla-Cortés Luis, Gheorghita Giulia Roxana, Currò Francesco, Calamandrei Rebecca, Susini Bianca, Callozzo Sara, Crivello Giulia, Russomanno Pasquale, Ravera Enrico, Cerofolini Linda, Fragai Marco
Department of Chemistry Ugo Schiff (DICUS), University of Florence, Via della Lastruccia 3, 50019 Sesto Fiorentino, Italy.
Centro di Risonanze Magnetiche (CERM), University of Florence, Via Luigi Sacconi 6, 50019 Sesto Fiorentino, Italy.
J Am Chem Soc. 2025 Jul 30;147(30):26379-26388. doi: 10.1021/jacs.5c05617. Epub 2025 Jul 16.
An effective delivery system is crucial for ensuring the therapeutic efficacy of a drug. This is especially true for biological drugs, which possess unique physicochemical properties and complex pharmacokinetic profiles, and thus require a dedicated design. Whole erythrocytes, and more recently, nanoparticles derived from red blood cells (RBCs), have been used in preclinical studies to deliver biological therapeutics: their biocompatibility and extended circulation time help prevent immunogenicity and reduce clearance and toxicity. However, characterizing such complex systems poses challenges that complicate their development and optimization. We argue that NMR spectroscopy enables the monitoring of the preservation of the high order structure of the encapsulated proteins, as well as their concentration, thereby assisting in formulation design, development, and manufacturing.
一个有效的递送系统对于确保药物的治疗效果至关重要。对于生物药物而言尤其如此，生物药物具有独特的物理化学性质和复杂的药代动力学特征，因此需要专门的设计。全红细胞以及最近从红细胞（RBC）衍生而来的纳米颗粒已用于临床前研究以递送生物治疗剂：它们的生物相容性和延长的循环时间有助于防止免疫原性并减少清除率和毒性。然而，表征此类复杂系统带来了挑战，使它们的开发和优化变得复杂。我们认为核磁共振波谱能够监测包封蛋白的高阶结构的保留情况及其浓度，从而有助于制剂的设计、开发和制造。
