无法诱导胸苷激酶活性的噬菌体T4突变体的分离。
Isolation of mutants of bacteriophage T4 unable to induce thymidine kinase activity.
作者信息
Chace K V, Hall D H
出版信息
J Virol. 1973 Aug;12(2):343-8. doi: 10.1128/JVI.12.2.343-348.1973.
Abstract
New mutants of T4 have been isolated by using a strain of Escherichia coli lacking thymidine kinase activity. These T4 mutants, designated tk, are able to grow on this E. coli strain under light on plates containing 5-bromodeoxyuridine and were all found to be unable to induce thymidine kinase (ATP: thymidine 5'-phosphotransferase, EC 2.7.1.21). All of these tk mutants fall into one complementation group which maps just to the right of rI on the standard T4 genetic map, far from most other genes coding for enzymes involved in pyrimidine metabolism. The tk mutants grow as well as wild-type T4, indicating that thymidine kinase is a non-essential enzyme.
摘要
通过使用一种缺乏胸苷激酶活性的大肠杆菌菌株，已分离出T4的新突变体。这些被命名为tk的T4突变体，在含有5-溴脱氧尿苷的平板上光照条件下，能够在这种大肠杆菌菌株上生长，并且都被发现无法诱导胸苷激酶（ATP：胸苷5'-磷酸转移酶，EC 2.7.1.21）。所有这些tk突变体都属于一个互补群，在标准T4遗传图谱上位于rI的右侧，远离大多数其他编码参与嘧啶代谢的酶的基因。tk突变体的生长情况与野生型T4相同，这表明胸苷激酶是一种非必需酶。
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