噬菌体T4 58.3至65.5千碱基早期区域的核苷酸序列及分析
Nucleotide sequence and analysis of the 58.3 to 65.5-kb early region of bacteriophage T4.
作者信息
Valerie K, Stevens J, Lynch M, Henderson E E, de Riel J K
出版信息
Nucleic Acids Res. 1986 Nov 11;14(21):8637-54. doi: 10.1093/nar/14.21.8637.
Abstract
The complete 7.2-kb nucleotide sequence from the 58.3 to 65.5-kb early region of bacteriophage T4 has been determined by Maxam and Gilbert sequencing. Computer analysis revealed at least 20 open reading frames (ORFs) within this sequence. All major ORFs are transcribed from the left strand, suggesting that they are expressed early during infection. Among the ORFs, we have identified the ipIII, ipII, denV and tk genes. The ORFs are very tightly spaced, even overlapping in some instances, and when ORF interspacing occurs, promoter-like sequences can be implicated. Several of the sequences preceding the ORFs, in particular those at ipIII, ipII, denV, and orf61.9, can potentially form stable stem-loop structures.
摘要
通过马克萨姆和吉尔伯特测序法确定了噬菌体T4早期区域58.3至65.5kb处完整的7.2kb核苷酸序列。计算机分析显示该序列内至少有20个开放阅读框（ORF）。所有主要的ORF均从左链转录，这表明它们在感染早期表达。在这些ORF中，我们鉴定出了ipIII、ipII、denV和tk基因。这些ORF间隔非常紧密，在某些情况下甚至重叠，当出现ORF间隔时，可能涉及启动子样序列。几个ORF之前的序列，特别是ipIII、ipII、denV和orf61.9处的序列，有可能形成稳定的茎环结构。
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本文引用的文献
1
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Virology. 1963 Jan;19:66-71. doi: 10.1016/0042-6822(63)90025-4.
2
Codon preference and its use in identifying protein coding regions in long DNA sequences.密码子偏好性及其在长DNA序列中识别蛋白质编码区的应用。
Nucleic Acids Res. 1982 Jan 11;10(1):141-56. doi: 10.1093/nar/10.1.141.
3
Plasmid screening at high colony density.高菌落密度下的质粒筛选
Gene. 1980 Jun;10(1):63-7. doi: 10.1016/0378-1119(80)90144-4.
4
Mutations in a nonessential viral gene permit bacteriophage T4 to form plaques on Escherichia coli valS ts relA.非必需病毒基因中的突变使噬菌体T4能够在大肠杆菌valS ts relA上形成噬菌斑。
Science. 1980 Jul 11;209(4453):294-5. doi: 10.1126/science.6992274.
5
Identification of the origins of T4 DNA replication.T4噬菌体DNA复制起始点的鉴定。
Proc Natl Acad Sci U S A. 1982 Dec;79(23):7248-52. doi: 10.1073/pnas.79.23.7248.
6
Intervening sequence in the thymidylate synthase gene of bacteriophage T4.噬菌体T4胸苷酸合成酶基因中的间隔序列。
Proc Natl Acad Sci U S A. 1984 May;81(10):3049-53. doi: 10.1073/pnas.81.10.3049.
7
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Mol Gen Genet. 1984;194(1-2):232-40. doi: 10.1007/BF00383522.
8
Transcription from efficient promoters can interfere with plasmid replication and diminish expression of plasmid specified genes.来自高效启动子的转录可能会干扰质粒复制并降低质粒特定基因的表达。
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Nucleic Acids Res. 1983 Nov 25;11(22):8019-30. doi: 10.1093/nar/11.22.8019.
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