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携带噬菌体T4胸苷激酶基因的γ-T4杂交噬菌体。

Gamma-T4 hybrid bacteriophage carrying the thymidine kinase gene of bacteriophage T4.

作者信息

Mileham A J, Murray N E, Revel H R

出版信息

J Virol. 1984 May;50(2):619-22. doi: 10.1128/JVI.50.2.619-622.1984.

DOI:10.1128/JVI.50.2.619-622.1984
PMID:6323761
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC255686/
Abstract

Among 32 lambda-T4 recombinant phages selected for growth on a thymidylate synthetase-deficient (thyA) host, 2 were shown to carry the T4 thymidine kinase (tk) gene. The lambda-T4tk phages contain two T4 HindIII DNA fragments (2.0 and 1.5 kilobases) that hybridize to restriction fragments of T4 DNA, encompassing the tk locus at 60 kilobases on the T4 map. The T4tk insert compensates for the simultaneous host deficiencies of thymidine kinase and thymidylate synthetase in a thymidine kinase-deficient (tdk) host growing in the presence of fluorodeoxyuridine when provided with thymidine and uridine. The lambda-T4tk hybrid phages specified five polypeptides with Mrs of 22,000 (22K), 21K, 14K, 11K, and 9K.

摘要

在为在胸苷酸合成酶缺陷型（thyA）宿主上生长而挑选出的32个λ-T4重组噬菌体中，有2个被证明携带T4胸苷激酶（tk）基因。λ-T4tk噬菌体包含两个T4 HindIII DNA片段（2.0和1.5千碱基），它们与T4 DNA的限制性片段杂交，在T4图谱上位于60千碱基处包含tk基因座。当向在氟脱氧尿苷存在下生长的胸苷激酶缺陷型（tdk）宿主提供胸苷和尿苷时，T4tk插入片段可弥补胸苷激酶和胸苷酸合成酶同时存在的宿主缺陷。λ-T4tk杂交噬菌体指定了5种分子量分别为22,000（22K）、21K、14K、11K和9K的多肽。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0812/255686/15ffd1a8a4fd/jvirol00134-0343-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0812/255686/15ffd1a8a4fd/jvirol00134-0343-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0812/255686/15ffd1a8a4fd/jvirol00134-0343-a.jpg

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本文引用的文献

1
THE MODE OF ACTION OF 5-FLUOROURACIL AND ITS DERIVATIVES.5-氟尿嘧啶及其衍生物的作用模式。
Proc Natl Acad Sci U S A. 1958 Oct 15;44(10):1004-12. doi: 10.1073/pnas.44.10.1004.
2
INABILITY OF THYMINE-DEPENDENT MUTANTS OF BACTERIOPHAGE T4 TO INDUCE THYMIDYLATE SYNTHETASE.噬菌体T4的胸腺嘧啶依赖性突变体诱导胸苷酸合成酶的无能性。
Proc Natl Acad Sci U S A. 1965 Apr;53(4):874-81. doi: 10.1073/pnas.53.4.874.
3
THYMIDINE-REQUIRING MUTANTS OF PHAGE T4.噬菌体T4的胸腺嘧啶核苷需求型突变体
Nucleic Acids Res. 1986 Nov 11;14(21):8637-54. doi: 10.1093/nar/14.21.8637.
Proc Natl Acad Sci U S A. 1963 Sep;50(3):526-32. doi: 10.1073/pnas.50.3.526.
4
Molecular cloning of the T4 genome: organization and expression of the tail fiber gene cluster 34--38.T4噬菌体基因组的分子克隆：尾丝基因簇34 - 38的组织与表达
Mol Gen Genet. 1981;182(3):445-55. doi: 10.1007/BF00293934.
5
Nucleotide sequence of the lysozyme gene of bacteriophage T4. Analysis of mutations involving repeated sequences.噬菌体T4溶菌酶基因的核苷酸序列。涉及重复序列的突变分析。
J Mol Biol. 1983 Apr 5;165(2):229-48. doi: 10.1016/s0022-2836(83)80255-1.
6
Physical mapping and cloning of bacteriophage T4 anti-restriction endonuclease gene.噬菌体T4抗限制内切核酸酶基因的物理图谱绘制与克隆
J Bacteriol. 1982 Feb;149(2):694-9. doi: 10.1128/jb.149.2.694-699.1982.
7
A restriction map of the bacteriophage T4 genome.噬菌体T4基因组的限制酶切图谱。
Mol Gen Genet. 1980;179(2):421-435. doi: 10.1007/BF00425473.
8
Molecular cloning of the T4 genome; organization and expression of the frd--DNA ligase region.T4噬菌体基因组的分子克隆；frd-DNA连接酶区域的组织与表达
Mol Gen Genet. 1980;179(2):227-39. doi: 10.1007/BF00425449.
9
A deoxythymidine kinase deficient mutant of Escherichia coli. II. Mapping and transduction studies with phage phi 80.大肠杆菌的一种脱氧胸苷激酶缺陷型突变体。II. 用噬菌体φ80进行的定位和转导研究
Genetics. 1967 Nov;57(3):643-54. doi: 10.1093/genetics/57.3.643.
10
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J Virol. 1973 Aug;12(2):343-8. doi: 10.1128/JVI.12.2.343-348.1973.