Chapman G S, Jones A L, Meyer U A, Bissell D M
J Cell Biol. 1973 Dec;59(3):735-47. doi: 10.1083/jcb.59.3.735.
Hepatic parenchymal cells from adult rats, established in vitro as a monolayer, have been evaluated by electron microscopy. Within 24 h after the initial seeding, the incubated cells were polygonal and in close apposition with three to six neighboring cells. The ultrastructure of the monolayer cells was examined at this time and after 3 and 10 days of incubation. With the exception of a few enlarged mitochondria, organelles in both the 1- and 3-day monolayer cells were indistinguishable quantitatively and morphologically from those found in the intact liver. After 10 days of incubation, however, the rough-surfaced endoplasmic reticulum (RER) had become dilated and vesiculated. In all cells studied, portions of RER were found in a close spatial relationship to mitochondria. From its frequency, this association appeared to be more than fortuitous, and the organelle complex may represent a functional unit necessary for new membrane formation, as suggested previously. The Golgi complexes of 1- and 3-day cells contained very low density lipoprotein-sized particles, which suggests that the monolayer cells synthesize lipoproteins. These electron microscope observations demonstrate that adult hepatic parenchymal cells in monolayer retain for several days the subcellular structural elements characteristic of normally functioning hepatocytes.
已通过电子显微镜对成年大鼠的肝实质细胞进行体外单层培养并评估。在最初接种后的24小时内，培养的细胞呈多边形，与三到六个相邻细胞紧密相邻。此时以及培养3天和10天后，对单层细胞的超微结构进行了检查。除了少数线粒体增大外，培养1天和3天的单层细胞中的细胞器在数量和形态上与完整肝脏中的细胞器没有区别。然而，培养10天后，糙面内质网（RER）变得扩张并形成囊泡。在所有研究的细胞中，发现部分RER与线粒体在空间上关系密切。从其出现频率来看，这种关联似乎并非偶然，并且如先前所提示的，细胞器复合体可能代表新膜形成所必需的功能单位。培养1天和3天的细胞的高尔基体复合体含有极低密度脂蛋白大小的颗粒，这表明单层细胞合成脂蛋白。这些电子显微镜观察结果表明，单层培养的成年肝实质细胞在数天内保留了正常功能肝细胞的亚细胞结构特征。
