Melmon K L, Weinstein Y, Shearer G M, Bourne H R, Bauminger S
J Clin Invest. 1974 Jan;53(1):22-30. doi: 10.1172/JCI107542.
Spleen cells from mice immunized with sheep red cells were separated by differential adherence to insolubilized histamine, catecholamines, and prostaglandins. The hormones were insolubilized by linking them to Sepharose beads through a protein carrier. We measured hemolytic plaque formation (per million splenic leukocytes) of cells which passed through columns of hormone-carrier-Sepharose beads (i.e., those cells that failed to bind). As compared with control (no column) cells, the number of plaque-forming cells was substantially reduced by passage through histamine, epinephrine, isoproterenol, and prostaglandin-E(2) columns. Plaque-forming cells were not significantly reduced by passage through carrier Sepharose (another control) or norepinephrine- and prostaglandin-F(2alpha)-carrier Sepharose columns. Thus, the ability of an insolubilized hormone preparation to subtract plaque-forming cells roughly correlated with the presence of pharmacologic receptors for the corresponding free hormones, as judged by stimulation of cyclic AMP accumulation in the same cells, reported previously. Both 19S and 7S plaque-forming cells were subtracted by columns prepared from pharmacologically active hormones, but none of the insolubilized hormones stimulated accumulation of intracellular cyclic AMP. The cell membrane phenomenon that allows adherence to a given hormone-carrier-bead column may be identical with the cell receptor.
用绵羊红细胞免疫的小鼠脾细胞通过对固定化组胺、儿茶酚胺和前列腺素的差异黏附进行分离。这些激素通过蛋白质载体与琼脂糖珠相连而被固定化。我们测量了通过激素 - 载体 - 琼脂糖珠柱的细胞（即未结合的细胞）的溶血空斑形成（每百万脾白细胞）。与对照（无柱）细胞相比，通过组胺、肾上腺素、异丙肾上腺素和前列腺素 - E(2)柱后，空斑形成细胞的数量大幅减少。通过载体琼脂糖柱（另一个对照）或去甲肾上腺素和前列腺素 - F(2α)-载体琼脂糖柱后，空斑形成细胞数量没有显著减少。因此，如先前报道通过同一细胞中环磷酸腺苷积累的刺激所判断，固定化激素制剂减去空斑形成细胞的能力大致与相应游离激素的药理受体的存在相关。19S和7S空斑形成细胞都被由药理活性激素制备的柱减去，但没有一种固定化激素刺激细胞内环磷酸腺苷的积累。允许黏附到给定激素 - 载体 - 珠柱上的细胞膜现象可能与细胞受体相同。
