高甲基化对转移核糖核酸功能特性的影响。核糖体结合与多肽合成。
The effect of hypermethylation on the functional properties of transfer ribonucleic acid. Ribosome-binding and polypeptide synthesis.
作者信息
Hay J, Pillinger D J, Borek E
出版信息
Biochem J. 1970 Sep;119(3):587-93. doi: 10.1042/bj1190587.
Abstract
- Phenylalanyl-tRNA formed after chemical hypermethylation of Escherichia coli B tRNA was able to bind to ribosomes with the same efficiency as normal phenylalanyl-tRNA. 2. Under incubation conditions used in the ribosome-binding assay, hypermethylation of tRNA did not measurably decrease the stability of either inter-nucleotide phosphodiester bonds or the covalent bond between amino acid and tRNA in phenylalanyl-tRNA. 3. The ability of hypermethylated tRNA to take part in polyphenylalanine synthesis was inhibited progressively as the degree of hypermethylation increased. 4. Hypermethylation of tRNA affected polyphenylalanine synthesis at the stage of amino acid recognition and at a further point in the synthesis but not at the level of codon-anticodon recognition. 5. The formation of polylysine was more seriously affected by hypermethylation of tRNA than would be accounted for by inhibition of amino acid acceptance alone. 6. Polyproline formation was completely inhibited by the presence of 7mol% excess of methyl groups in tRNA. 7. The possibility of a link between amino acid acceptance and ribosome-binding was suggested for phenylalanyl-tRNA, but not for lysyl- or prolyl-tRNA.
摘要
- 大肠杆菌B型tRNA经化学超甲基化后形成的苯丙氨酰-tRNA能够以与正常苯丙氨酰-tRNA相同的效率与核糖体结合。2. 在核糖体结合测定所用的孵育条件下，tRNA的超甲基化并未显著降低苯丙氨酰-tRNA中核苷酸间磷酸二酯键或氨基酸与tRNA之间共价键的稳定性。3. 随着超甲基化程度的增加，超甲基化tRNA参与多聚苯丙氨酸合成的能力逐渐受到抑制。4. tRNA的超甲基化在氨基酸识别阶段以及合成的进一步阶段影响多聚苯丙氨酸合成，但不影响密码子-反密码子识别水平。5. tRNA的超甲基化对多聚赖氨酸形成的影响比仅通过抑制氨基酸接纳所能解释的更为严重。6. tRNA中存在7mol%过量的甲基会完全抑制多聚脯氨酸的形成。7. 对于苯丙氨酰-tRNA，提示了氨基酸接纳与核糖体结合之间存在联系，但对于赖氨酰-tRNA或脯氨酰-tRNA则不然。
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