脑心肌炎病毒核糖核酸上的聚腺苷酸
Poly(A) on mengovirus RNA.
作者信息
Spector D H, Baltimore D
出版信息
J Virol. 1975 Oct;16(4):1081-4. doi: 10.1128/JVI.16.4.1081-1084.1975.
Abstract
The content and size of the poly(A) on Mengovirus RNA grown in both mouse L cells and HeLa cells have been examined. Virion RNA from either cell line could bind to poly(U) filters and contained RNase-resistant stretches of poly(A) which could be analyzed by electrophoresis in polyacrylamide gels. The size of the poly(A) on the Mengovirus RNA was independent of the host cell and averaged from 50 to 70 nucleotides.
摘要
对在小鼠L细胞和HeLa细胞中生长的脑心肌炎病毒RNA上的聚腺苷酸（poly(A)）的含量和大小进行了检测。来自这两种细胞系的病毒粒子RNA都能与聚尿苷酸（poly(U)）滤膜结合，并且含有对核糖核酸酶（RNase）有抗性的聚腺苷酸片段，这些片段可通过聚丙烯酰胺凝胶电泳进行分析。脑心肌炎病毒RNA上聚腺苷酸的大小与宿主细胞无关，平均为50至70个核苷酸。
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