超螺旋质粒DNA中RNA的大小和碱基组成
Size and base composition of RNA in supercoiled plasmid DNA.
作者信息
Williams H, Boyer H W, Helsinki D R
出版信息
Proc Natl Acad Sci U S A. 1973 Dec;70(12):3744-8. doi: 10.1073/pnas.70.12.3744.
Abstract
The average size and base composition of the covalently integrated RNA segment in supercoiled ColE(1) DNA synthesized in Escherichia coli in the presence of chloramphenicol (CM-ColE(1) DNA) have been determined by two independent methods. The two approaches yielded similar results, indicating that the RNA segment in CM-ColE(1) DNA contains GMP at the 5' end and comprises on the average 25 to 26 ribonucleotides with a base composition of 10-11 G, 3 A, 5-6 C, and 6-7 U.
摘要
在氯霉素存在的情况下于大肠杆菌中合成的超螺旋ColE(1) DNA（CM-ColE(1) DNA）中，共价整合的RNA片段的平均大小和碱基组成已通过两种独立方法确定。这两种方法得出了相似的结果，表明CM-ColE(1) DNA中的RNA片段在5'端含有鸟苷酸，平均由25至26个核糖核苷酸组成，碱基组成为10 - 11个鸟嘌呤、3个腺嘌呤、5 - 6个胞嘧啶和6 - 7个尿嘧啶。
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