体外RNA连接的DNA片段
RNA-linked DNA fragments in vitro.
作者信息
Sugino A, Okazaki R
出版信息
Proc Natl Acad Sci U S A. 1973 Jan;70(1):88-92. doi: 10.1073/pnas.70.1.88.
Abstract
RNA-linked DNA fragments are intermediates in DNA replication in Escherichia coli cells made permeable to nucleoside triphosphates by treatment with toluene. Covalent linkage of a short RNA stretch to the 5' end of the DNA is proved by transfer of (32)P from [alpha-(32)P]dNTP to ribonucleotides upon digestion with alkali or pancreatic RNase, and by a small decrease in the molecular size upon alkaline hydrolysis. The (32)P transfer experiments reveal a unique structure...p(rPy)p(rA)p(rU or rC)p(dC)p... at the RNA-DNA junction.
摘要
RNA连接的DNA片段是经甲苯处理后可渗透核苷三磷酸的大肠杆菌细胞中DNA复制的中间体。通过用碱或胰核糖核酸酶消化后，[α-(32)P]dNTP中的(32)P转移至核糖核苷酸，以及通过碱性水解后分子大小的小幅减小，证明了短RNA片段与DNA 5'端的共价连接。(32)P转移实验揭示了RNA-DNA连接处的独特结构……p(rPy)p(rA)p(rU或rC)p(dC)p……
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Slow joining of newly replicated DNA chains in DNA polymerase I-deficient Escherichia coli mutants.DNA聚合酶I缺陷型大肠杆菌突变体中新复制DNA链的缓慢连接。
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Replication and repair of DNA in cells of Escherichia coli treated with toluene.用甲苯处理的大肠杆菌细胞中DNA的复制与修复
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A possible role for RNA polymerase in the initiation of M13 DNA synthesis.RNA聚合酶在M13 DNA合成起始过程中的可能作用。
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