Perry D
J Bacteriol. 1974 Feb;117(2):702-7. doi: 10.1128/jb.117.2.702-707.1974.
The binding of streptococcal competence factor (CF) was found to be specific for a strain of streptococcus which was capable of undergoing competence induction. Three other streptococcal strains which could not be induced to competence, did not bind CF. CF binding was independent of time, temperature, age of the culture, and type of growth media employed. Several observations indicated that the receptor sites for CF are located in the bacterial membrane: (i) the retention of CF by spheroplasts, (ii) the binding of CF by isolated membrane fractions, and (iii), the degradation of CF binding capacity of membranes by different chemicals and enzymes.
发现链球菌感受态因子(CF)的结合对于能够进行感受态诱导的一种链球菌菌株具有特异性。另外三种不能被诱导形成感受态的链球菌菌株不结合CF。CF结合与时间、温度、培养物的菌龄以及所用生长培养基的类型无关。多项观察结果表明CF的受体位点位于细菌细胞膜中:(i)原生质体对CF的保留,(ii)分离的膜组分对CF的结合,以及(iii)不同化学物质和酶对膜CF结合能力的降解。