Pakula R
J Bacteriol. 1967 Jul;94(1):75-9. doi: 10.1128/jb.94.1.75-79.1967.
Immune globulins against competent cells of group H streptococci, strains Challis and Wicky, inhibited genetic transformation to streptomycin resistance when added to competent cultures. Antibodies against noncompetent cells did not inhibit transformation of competent cells. Strain Challis is spontaneously highly transformable. Strain Wicky is very poorly transformable but can be converted to high transformability with the exocellular competence-provoking factor (CPF) produced by strain Challis. Globulins against noncompetent cells of strain Challis and Wicky also inhibited transformation when added to noncompetent cultures prior to conversion to competence. Antibodies against cells of the related strain Blackburn, however, did not inhibit transformation under any circumstances. It is concluded that, although globulins prepared against competent cells block the deoxyribonucleic acid receptor sites present in these cells, the globulins prepared against noncompetent cells prevent conversion to competence by blocking the access of CPF to specific cellular sites for this factor. Strain Blackburn seems not to contain CPF-receptive sites and is, therefore, nontransformable.
针对H群链球菌Challis株和Wicky株感受态细胞的免疫球蛋白，在添加到感受态培养物中时，可抑制向链霉素抗性的遗传转化。针对非感受态细胞的抗体不会抑制感受态细胞的转化。Challis株可自发地高度可转化。Wicky株的可转化性很差，但可通过Challis株产生的胞外感受态激发因子（CPF）转化为高可转化性。在转化为感受态之前，将针对Challis株和Wicky株非感受态细胞的球蛋白添加到非感受态培养物中时，也会抑制转化。然而，针对相关Blackburn株细胞的抗体在任何情况下都不会抑制转化。得出的结论是，尽管针对感受态细胞制备的球蛋白会阻断这些细胞中存在的脱氧核糖核酸受体位点，但针对非感受态细胞制备的球蛋白会通过阻断CPF进入该因子的特定细胞位点来阻止转化为感受态。Blackburn株似乎不含有CPF受体位点，因此不可转化。
