Taniguchi T, Fujii-Kuriyama Y, Muramatsu M
Proc Natl Acad Sci U S A. 1980 Jul;77(7):4003-6. doi: 10.1073/pnas.77.7.4003.
A hybrid plasmid, TpIF319, has been shown to contain the sequence for human fibroblast interferon mRNA [Taniguchi, T., Sakai, M., Fujii-Kuriyama, Y., Muramatsu, M., Kobayashi, S. & Sudo, T. (1979) Proc. Jpn. Acad. 55, Ser. B, 464-469]. This conclusion was confirmed by a hybridization-translation assay, using rabbit globin mRNA and its cDNA-containing plasmid as a control. Plasmid TpIF319 was used as probe and another recombinant plasmid, TpIF319-13, whose cDNA insert consists of about 800 base pairs, was isolated. Nucleotide sequence analysis of the cDNA revealed that the DNA in fact codes for human fibroblast interferon.
一种杂种质粒TpIF319已被证明含有人类成纤维细胞干扰素信使核糖核酸的序列[谷口哲、酒井正、藤井栗山洋、村松正、小林诚和须藤敏（1979年）《日本科学院学报》55卷，B辑，464 - 469页]。这一结论通过杂交 - 翻译试验得到了证实，该试验以兔珠蛋白信使核糖核酸及其含互补脱氧核糖核酸的质粒作为对照。质粒TpIF319用作探针，另一种重组质粒TpIF319 - 13被分离出来，其互补脱氧核糖核酸插入片段由约800个碱基对组成。对该互补脱氧核糖核酸的核苷酸序列分析表明，该脱氧核糖核酸实际上编码人类成纤维细胞干扰素。
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