仓鼠成纤维细胞外部蛋白质的特性分析
Characterization of the external proteins of hamster fibroblasts.
作者信息
Hynes R O, Humphryes K C
出版信息
J Cell Biol. 1974 Aug;62(2):438-48. doi: 10.1083/jcb.62.2.438.
Abstract
The results of metabolic labeling studies and enzymatic treatments followed by analysis on polyacrylamide gels show that the external proteins of hamster fibroblast cell lines, which have been identified by lactoperoxidase-catalyzed iodination, do not contain sulphated mucopolysaccharides or hyaluronic acid and are probably unrelated to collagen. Several of the iodinated species comigrate with carbohydrate-containing molecules. In particular, the major iodine-labeled polypeptide of normal fibroblasts appears to be a glycoprotein. This glycoprotein is absent or much reduced in virus-transformed cells, as detected both by iodination and by metabolic labeling. We conclude that the major iodinated polypeptide is not detected on transformed cells because it is absent rather than because it is masked. Approximate molecular weights of the external proteins are also reported.
摘要
代谢标记研究以及随后在聚丙烯酰胺凝胶上进行分析的酶处理结果表明，经乳过氧化物酶催化碘化鉴定的仓鼠成纤维细胞系的外部蛋白质不含硫酸化粘多糖或透明质酸，可能与胶原蛋白无关。几种碘化物种与含碳水化合物的分子一起迁移。特别是，正常成纤维细胞的主要碘标记多肽似乎是一种糖蛋白。通过碘化和代谢标记检测发现，这种糖蛋白在病毒转化细胞中不存在或大量减少。我们得出结论，在转化细胞上未检测到主要的碘化多肽是因为它不存在而不是因为它被掩盖。还报告了外部蛋白质的近似分子量。
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