Brysk M M, Snider J M
J Invest Dermatol. 1982 Jan;78(1):24-7. doi: 10.1111/1523-1747.ep12497859.
Cell surface proteins of normal human, mouse, and rat cells in primary culture, of human basal cell carcinoma, and of carcinogen-transformed cell lines were examined by lactoperoxidase-catalyzed iodination. Autoradiography was used to record the distribution of label in the polypeptide subunits separated by sodium dodecyl sulfate polyacrylamide gel electrophoresis. There was no significant difference in the results for normal cells of human, mouse, and rat. On the other hand, carcinogen-transformed mouse cells had many more labeled polypeptide bands of widely distributed molecular weights. The iodination profiles from human basal cell carcinoma cells were much more akin to those from normal cells than to those from carcinogen-transformed cells. Treatment of iodinated cells with proteolytic enzymes visibly altered the polypeptide bands.
利用乳过氧化物酶催化碘化法对原代培养的正常人、小鼠和大鼠细胞、人基底细胞癌以及致癌物转化细胞系的细胞表面蛋白进行了检测。采用放射自显影法记录经十二烷基硫酸钠聚丙烯酰胺凝胶电泳分离的多肽亚基中标记物的分布情况。人、小鼠和大鼠正常细胞的检测结果无显著差异。另一方面，致癌物转化的小鼠细胞有更多分子量分布广泛的标记多肽条带。人基底细胞癌细胞的碘化图谱与正常细胞的图谱更为相似，而与致癌物转化细胞的图谱差异较大。用蛋白水解酶处理碘化细胞后，多肽条带明显改变。
