在体内给予致癌物N-亚硝基二甲胺后大鼠肝脏脱氧核糖核酸中的磷酸三酯。
Phosphotriesters in rat liver deoxyribonucleic acid after the administration of the carcinogen NN-dimethylnitrosamine in vivo.
作者信息
O'Connor P J, Marigison G P, Craig A W
出版信息
Biochem J. 1975 Mar;145(3):475-82. doi: 10.1042/bj1450475.
Abstract
After treatment with NN-di[14C]methylnitrosamine, samples of DNA were isolated from rat livers by a conventional phenol procedure and examined for the presence of phosphotriesters. A method of capable of detecting relatively small amounts of 14C-labelled phosphotriesters was developed and used to establish that these products account for 10-12% of the total methylation pattern found after treatment with this agent in vitro. The significance of the presence of phosphotriesters in DNA is discussed.
摘要
用NN-二[14C]甲基亚硝胺处理后，通过传统的酚法从大鼠肝脏中分离出DNA样本，并检测其中磷酸三酯的存在情况。开发了一种能够检测相对少量14C标记磷酸三酯的方法，并用于确定这些产物占体外使用该试剂处理后发现的总甲基化模式的10-12%。讨论了DNA中磷酸三酯存在的意义。
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