核基因剂量对线粒体质量和DNA的影响。
Nuclear gene dosage effects on mitochondrial mass and DNA.
作者信息
Grimes G W, Mahler H R, Perlman R S
出版信息
J Cell Biol. 1974 Jun;61(3):565-74. doi: 10.1083/jcb.61.3.565.
In order to assess the effect of nuclear gene dosage on the regulation of mitochondria we have studied serial sections of a set of isogenic haploid and diploid cells of Saccharomyces cerevisiae, growing exponentially in the absence of catabolite repression, and determined the amount of mitochondrial DNA per cell. Mitochondria accounted for 14% of the cytoplasmic and 12% of the total cellular volume in all cells examined regardless of their ploidy or their apparent stage in the cell cycle. The mean number of mitochondria per cell was 22 in the diploid and 10 in the haploids. The volume distribution appeared unimodal and identical in haploids and diploids. The mitochondrial DNA accounted for 12.6 +/- 1.2% and 13.5 +/- 1.3% of the total cellular DNA in the diploid and haploid populations, respectively. These values correspond to 3.6 x 10(-15) g, 2.2 x 10(9) daltons, or 44 genomes (50 x 10(6) daltons each) per haploid and twice that per diploid cell. On this basis, the average mitochondrion in these cells contains four mitochondrial genomes in both the haploid and the diploid.
为了评估核基因剂量对线粒体调控的影响，我们研究了一组酿酒酵母同基因单倍体和二倍体细胞的连续切片，这些细胞在没有分解代谢物阻遏的情况下呈指数生长，并测定了每个细胞中线粒体DNA的含量。在所检查的所有细胞中，无论其倍性或在细胞周期中的明显阶段如何，线粒体占细胞质的14%，占细胞总体积的12%。每个二倍体细胞中线粒体的平均数量为22个，单倍体细胞中为10个。单倍体和二倍体的体积分布呈现单峰且相同。线粒体DNA分别占二倍体和单倍体群体中细胞总DNA的12.6±1.2%和13.5±1.3%。这些值分别对应于每个单倍体细胞3.6×10⁻¹⁵克、2.2×10⁹道尔顿或44个基因组（每个50×10⁶道尔顿），每个二倍体细胞的量是单倍体细胞的两倍。在此基础上，这些细胞中的平均每个线粒体在单倍体和二倍体中都含有四个线粒体基因组。
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