Pringle C R, Wunner W H
J Virol. 1973 Oct;12(4):677-83. doi: 10.1128/JVI.12.4.677-683.1973.
Temperature-sensitive (ts) mutants of Cocal virus (VSV Cocal) were isolated after treatment with the base analogue mutagen, 5-fluorouracil. These mutants could be classified into four mutually complementing groups. Weak complementation was detected between certain pairs of VSV Cocal ts mutants and ts mutants of vesicular stomatitis virus (VSV) Indiana, but no complementation was observed with ts mutants of VSV New Jersey. Two complementing ts mutants of Chandipura virus, an unrelated rhabdovirus, did not complement any VSV mutant, Thus, ability to complement in the VSV group appears to be correlated with serological relationships. The RNA and protein-synthesizing capacities of these ts mutants have been determined, and it is possible to establish a correspondence between the VSV Cocal and the VSV Indiana complementation groups.
在用碱基类似物诱变剂5-氟尿嘧啶处理后,分离出了可卡病毒(VSV可卡)的温度敏感(ts)突变体。这些突变体可分为四个相互互补的组。在某些VSV可卡ts突变体对与水疱性口炎病毒(VSV)印第安纳株的ts突变体之间检测到弱互补,但未观察到与VSV新泽西株的ts突变体有互补现象。与VSV无关的弹状病毒钱迪普拉病毒的两个互补ts突变体,不与任何VSV突变体互补。因此,在VSV组中互补的能力似乎与血清学关系相关。已测定了这些ts突变体的RNA和蛋白质合成能力,并且有可能在VSV可卡和VSV印第安纳互补组之间建立对应关系。