Holland J J, Villarreal L P
Proc Natl Acad Sci U S A. 1974 Aug;71(8):2956-60. doi: 10.1073/pnas.71.8.2956.
Infectious B virions of vesicular stomatitis virus were 100% lethal to BHK(21) (baby hamster kidney) cells when infecting alone, and persistent noncytocidal infection could not be achieved with cloned B virions alone. However, a mixture of B virions and homologous, short, defective, interfering particles (T particles) of a temperature-sensitive mutant of the virus regularly established persistently infected, noncytocidal carrier cultures. A long T particle was generated during establishment of the carrier culture; we show that this long T particle can establish and maintain persistent noncytocidal infection even when it infects cells along with virulent wild-type B virions. This long T particle causes the production of wild-type B virions with greatly reduced virion transcriptase (EC 2.7.7.6; RNA nucleotidyltransferase) levels when coinfecting the same cells, so it appears to prevent cytopathology by regulating virus transcription. The implications of these findings for rabies and other slowly progressing noncytocidal infections are discussed.
单独感染时，水疱性口炎病毒的感染性B病毒粒子对BHK(21)（幼仓鼠肾）细胞具有100%的致死性，仅用克隆的B病毒粒子无法实现持续性非杀细胞感染。然而，B病毒粒子与该病毒温度敏感突变体的同源、短小、缺陷、干扰性颗粒（T颗粒）的混合物可定期建立持续性感染的非杀细胞载体培养物。在载体培养物建立过程中产生了一种长T颗粒；我们发现，即使这种长T颗粒与强毒野生型B病毒粒子一起感染细胞，它也能建立并维持持续性非杀细胞感染。当共同感染同一细胞时，这种长T颗粒会导致野生型B病毒粒子的病毒体转录酶（EC 2.7.7.6；RNA核苷酸转移酶）水平大幅降低，因此它似乎通过调节病毒转录来预防细胞病变。讨论了这些发现对狂犬病和其他缓慢进展的非杀细胞感染的意义。
