从感染腺病毒2的海拉细胞核中体外翻译总核RNA。
Translation in vitro of total nuclear RNA from HeLa cell nuclei infected with adenovirus 2.
作者信息
Chatterjee N K, Weissbach H
出版信息
Proc Natl Acad Sci U S A. 1974 Aug;71(8):3129-33. doi: 10.1073/pnas.71.8.3129.
Abstract
Total heterogeneous nuclear RNA from HeLa cells infected with adenovirus for 18-20 hr stimulates amino acid incorporation into protein in a cell-free system from Ehrlich ascites tumors. This stimulation of protein synthesis by the nuclear RNA requires intact nuclei. The role of nuclei in this system is unknown, but evidence is presented that the nuclei are involved in the conversion of high-molecular-weight RNA to low-molecular-weight species. Some of the newly synthesized polypeptides appear to resemble the virion polypeptides.
摘要
用腺病毒感染18 - 20小时的HeLa细胞的总异质核RNA，能刺激艾氏腹水瘤细胞的无细胞体系中氨基酸掺入蛋白质。核RNA对蛋白质合成的这种刺激作用需要完整的细胞核。细胞核在该体系中的作用尚不清楚，但有证据表明细胞核参与了高分子量RNA向低分子量RNA种类的转化。一些新合成的多肽似乎类似于病毒粒子多肽。
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本文引用的文献
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