Parsons J T, Gardner J, Green M
Proc Natl Acad Sci U S A. 1971 Mar;68(3):557-60. doi: 10.1073/pnas.68.3.557.
Late after infection of cultured human cells (KB) with adenovirus type 2, the nucleus contains heterogeneous viral RNA species ranging in size from 10 to 43 S. Four viral RNA species found in the nucleus (36, 38, 40, and 43 S) are synthesized predominantly during a 15-min labeling period with [(3)H]uridine, while smaller RNA species accumulate when labeling is continued for longer periods. In contrast, 6-8 viral RNA species, of sedimentation coefficient from 10 to 29 S, are found in the cytoplasm after a 30-min pulse label and a 2-hr chase. DNA-RNA hybridization-competition experiments demonstrate that viral RNA sequences present in nuclear 36-43S RNA are also present in cytoplasmic and polyribosomal RNA, suggesting that at least some of the cytoplasmic viral-specific RNA molecules are derived by cleavage of high molecular weight precursors from the nucleus.
在培养的人细胞（KB）被2型腺病毒感染后晚期，细胞核中含有大小从10到43 S不等的异质病毒RNA种类。在细胞核中发现的四种病毒RNA种类（36、38、40和43 S）主要是在15分钟的[³H]尿苷标记期合成的，而当标记持续更长时间时，较小的RNA种类会积累。相比之下，在30分钟脉冲标记和2小时追踪后，在细胞质中发现了6 - 8种沉降系数为10到29 S的病毒RNA种类。DNA - RNA杂交竞争实验表明，存在于细胞核36 - 43S RNA中的病毒RNA序列也存在于细胞质和多核糖体RNA中，这表明至少一些细胞质中的病毒特异性RNA分子是由细胞核中高分子量前体的切割产生的。
