用巨噬细胞游走抑制试验比较用肝癌免疫的豚鼠中肝癌细胞、正常肝细胞、胎儿肝细胞及化学损伤肝细胞的抗原性。
Comparison of antigenicity of hepatoma cells, normal liver cells, foetal liver cells and chemically damaged liver cells in guinea-pigs immunized with hepatomata using the macrophage migration inhibition test.
作者信息
Desai H N, Dale M M
出版信息
Br J Cancer. 1974 Aug;30(2):109-7. doi: 10.1038/bjc.1974.121.
The macrophage migration inhibition test has been used to study the immune responses of guinea-pigs immunized with injections of whole cells of both an allogeneic and a syngeneic hepatoma grown as established cell lines in tissue culture.A clear dose-response relationship between tumour cell concentration and migration inhibition was seen in immunized animals and no significant migration inhibition was seen in control animals. There was no cross reaction between the two tumours used. There was no cross reaction between whole isolated normal liver cells and tumour cells, or between foetal liver cells and tumour cells. Whole isolated liver cells from carbon tetrachloride damaged livers caused some degree of migration inhibition in both normal and immunized guinea-pigs but, taking this into account, they did not appear to cross react with hepatoma cells.
巨噬细胞游走抑制试验已被用于研究豚鼠的免疫反应，这些豚鼠通过注射在组织培养中作为已建立细胞系生长的同种异体和同基因肝癌的全细胞进行免疫。在免疫动物中观察到肿瘤细胞浓度与游走抑制之间存在明显的剂量反应关系，而在对照动物中未观察到明显的游走抑制。所使用的两种肿瘤之间没有交叉反应。分离的正常肝细胞与肿瘤细胞之间、胎儿肝细胞与肿瘤细胞之间均无交叉反应。来自四氯化碳损伤肝脏的分离的全肝细胞在正常和免疫豚鼠中均引起一定程度的游走抑制，但考虑到这一点，它们似乎与肝癌细胞没有交叉反应。
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