Matches J R, Liston J, Curran D
Appl Microbiol. 1974 Oct;28(4):655-60. doi: 10.1128/am.28.4.655-660.1974.
Clostridium perfringens was isolated from samples collected in Puget Sound in the state of Washington and areas considered as possible sources of these organisms to Puget Sound. The distribution of C. perfringens in the total Clostridium population was determined for fish gut contents and sediments collected in highly polluted and less polluted areas, sewage samples, freshwater sediments, and soils. The greatest numbers of C. perfringens were obtained from marine sediments collected near the sewage outfall at West Point. Fewer isolates were made from fish collected from less polluted stations, although the number of C. perfringens remained high in sediments from other Puget Sound stations. The proportion of C. perfringens in the total Clostridium populations varied between 56 and 71% for sewage samples and only 0.4 to 4.1% for freshwater sediments and soil samples. Only 25 C. perfringens isolates out of 137 from fish guts, or 18%, were identifiable serologically and these fell into 12 groups. C. perfringens were fed to fish and the fish were sacrificed after varying lengths of time. The number of C. perfringens increased slightly in the gut during the first 24 h and then the numbers decreased rapidly for the next 120 h.
从华盛顿州普吉特海湾采集的样本以及被认为可能是这些生物体进入普吉特海湾源头的区域中分离出了产气荚膜梭菌。测定了在污染严重和污染较轻地区采集的鱼肠道内容物和沉积物、污水样本、淡水沉积物及土壤中产气荚膜梭菌在梭菌总菌群中的分布情况。产气荚膜梭菌数量最多的样本来自西点污水处理厂附近采集的海洋沉积物。从污染较轻站点采集的鱼中分离出的菌株较少，不过在普吉特海湾其他站点的沉积物中，产气荚膜梭菌数量仍然很高。在污水样本中，产气荚膜梭菌在梭菌总菌群中的比例在56%至71%之间，而在淡水沉积物和土壤样本中仅为0.4%至4.1%。从137株鱼肠道产气荚膜梭菌分离株中，只有25株（即18%）可通过血清学鉴定，且这些菌株分属于12个组。将产气荚膜梭菌投喂给鱼，在不同时长后宰杀这些鱼。在最初24小时内，鱼肠道内产气荚膜梭菌数量略有增加，随后在接下来的120小时内迅速减少。
