小鼠 - 人杂交亚克隆中SV40 T抗原表达与人类7号染色体的一致分离。
Concordant segregation of the expression of SV40 T antigen and human chromosome 7 in mouse-human hybrid subclones.
作者信息
Croce C M, Koprowski H
出版信息
J Exp Med. 1974 May 1;139(5):1350-3. doi: 10.1084/jem.139.5.1350.
Abstract
Subcloning of Simian virus 40 (SV40) T antigen-positive mouse-human hybrids, derived from the fusion of mouse cells deficient in thymidine kinase with SV40-transformed Lesch Nyhan fibroblasts, resulted in their segregation into T antigen-positive and negative subclones. Positive correlation between the presence of human chromosome 7 and the expression of SV40 T antigen was established in the subclones examined. These results negate the possibility of a transfer of the SV40 genome to a mouse chromosome.
摘要
通过将缺乏胸苷激酶的小鼠细胞与SV40转化的莱施-尼汉成纤维细胞融合而获得的猿猴病毒40（SV40）T抗原阳性的小鼠-人杂种细胞进行亚克隆，结果它们被分离成T抗原阳性和阴性亚克隆。在所检测的亚克隆中确定了人类7号染色体的存在与SV40 T抗原表达之间的正相关。这些结果否定了SV40基因组转移到小鼠染色体上的可能性。
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