Lundquist R E, Ehrenfeld E, Maizel J V
Proc Natl Acad Sci U S A. 1974 Dec;71(12):4773-7. doi: 10.1073/pnas.71.12.4773.
Poliovirus-infected HeLa cells were labeled with radioactive methionine or phenylalanine and subjected to a new purification procedure for the viral induced RNA polymerase activity. Detergent-solubilized polymerase activity was purified by precipitation with 2 M LiCl and sedimentation through sucrose gradients. Approximately 0.001% of the incorporated amino acid radio-activity sediments with the peak of polymerase activity. Gradient fractions comprising the polymerase activity peak were analyzed by sodium dodecyl sulfate-polyacrylamide gel electrophoresis and found to contain predominantly one virus-specific polypeptide. Polyacrylamide gel electrophoresis also reveals that this purified polypeptide migrates with a 58,000 molecular weight noncapsid polio-virus polypeptide.
用放射性甲硫氨酸或苯丙氨酸标记脊髓灰质炎病毒感染的海拉细胞，并对病毒诱导的RNA聚合酶活性进行新的纯化程序。用2M LiCl沉淀并通过蔗糖梯度沉降来纯化去污剂增溶的聚合酶活性。掺入的氨基酸放射性约0.001%与聚合酶活性峰一起沉降。通过十二烷基硫酸钠-聚丙烯酰胺凝胶电泳分析包含聚合酶活性峰的梯度级分，发现主要含有一种病毒特异性多肽。聚丙烯酰胺凝胶电泳还显示，这种纯化的多肽与分子量为58,000的非衣壳脊髓灰质炎病毒多肽一起迁移。
