与脊髓灰质炎病毒RNA聚合酶相关的一种病毒多肽的分离
Isolation of a viral polypeptide associated with poliovirus RNA polymerase.
作者信息
Lundquist R E, Ehrenfeld E, Maizel J V
出版信息
Proc Natl Acad Sci U S A. 1974 Dec;71(12):4773-7. doi: 10.1073/pnas.71.12.4773.
Abstract
Poliovirus-infected HeLa cells were labeled with radioactive methionine or phenylalanine and subjected to a new purification procedure for the viral induced RNA polymerase activity. Detergent-solubilized polymerase activity was purified by precipitation with 2 M LiCl and sedimentation through sucrose gradients. Approximately 0.001% of the incorporated amino acid radio-activity sediments with the peak of polymerase activity. Gradient fractions comprising the polymerase activity peak were analyzed by sodium dodecyl sulfate-polyacrylamide gel electrophoresis and found to contain predominantly one virus-specific polypeptide. Polyacrylamide gel electrophoresis also reveals that this purified polypeptide migrates with a 58,000 molecular weight noncapsid polio-virus polypeptide.
摘要
用放射性甲硫氨酸或苯丙氨酸标记脊髓灰质炎病毒感染的海拉细胞，并对病毒诱导的RNA聚合酶活性进行新的纯化程序。用2M LiCl沉淀并通过蔗糖梯度沉降来纯化去污剂增溶的聚合酶活性。掺入的氨基酸放射性约0.001%与聚合酶活性峰一起沉降。通过十二烷基硫酸钠-聚丙烯酰胺凝胶电泳分析包含聚合酶活性峰的梯度级分，发现主要含有一种病毒特异性多肽。聚丙烯酰胺凝胶电泳还显示，这种纯化的多肽与分子量为58,000的非衣壳脊髓灰质炎病毒多肽一起迁移。
相似文献
1
Isolation of a viral polypeptide associated with poliovirus RNA polymerase.与脊髓灰质炎病毒RNA聚合酶相关的一种病毒多肽的分离
Proc Natl Acad Sci U S A. 1974 Dec;71(12):4773-7. doi: 10.1073/pnas.71.12.4773.
2
The formation of poliovirus particles in association with the RNA replication complexes.脊髓灰质炎病毒颗粒与RNA复制复合体的形成。
J Gen Virol. 1973 Oct;21:99-108. doi: 10.1099/0022-1317-21-1-99.
3
Poliovirus polyuridylic acid polymerase and RNA replicase have the same viral polypeptide.脊髓灰质炎病毒聚尿苷酸聚合酶和RNA复制酶具有相同的病毒多肽。
J Virol. 1979 Jan;29(1):352-60. doi: 10.1128/JVI.29.1.352-360.1979.
4
Purification, characterization, and comparison of poliovirus RNA polymerase from native and recombinant sources.来自天然和重组来源的脊髓灰质炎病毒RNA聚合酶的纯化、特性鉴定及比较。
J Biol Chem. 1991 Dec 15;266(35):24212-9.
5
Isolation and subunit structure of polycytidylate-dependent RNA polymerase of encephalomyocarditis virus.
Proc Natl Acad Sci U S A. 1972 Dec;69(12):3815-9. doi: 10.1073/pnas.69.12.3815.
6
Multiple isoelectric forms of poliovirus RNA-dependent RNA polymerase: evidence for phosphorylation.脊髓灰质炎病毒RNA依赖性RNA聚合酶的多种等电形式：磷酸化的证据
J Virol. 1989 Nov;63(11):4563-8. doi: 10.1128/JVI.63.11.4563-4568.1989.
7
Analysis of RNA associated with the poliovirus RNA replication complexes.与脊髓灰质炎病毒RNA复制复合体相关的RNA分析
Virology. 1974 Apr;58(2):526-35. doi: 10.1016/0042-6822(74)90086-5.
8
Isolation of a soluble and template-dependent poliovirus RNA polymerase that copies virion RNA in vitro.一种可溶且依赖模板的脊髓灰质炎病毒RNA聚合酶的分离，该聚合酶可在体外复制病毒粒子RNA。
J Virol. 1979 Oct;32(1):155-61. doi: 10.1128/JVI.32.1.155-161.1979.
9
Isolation and properties of an RNA polymerase from influenza virus-infected cells.从感染流感病毒的细胞中分离出RNA聚合酶及其特性
J Virol. 1973 Mar;11(3):441-8. doi: 10.1128/JVI.11.3.441-448.1973.
10
Identification of poliovirus polypeptide P63 as a soluble RNA-dependent RNA polymerase.脊髓灰质炎病毒多肽P63作为一种可溶性RNA依赖性RNA聚合酶的鉴定。
J Virol. 1980 Sep;35(3):732-40. doi: 10.1128/JVI.35.3.732-740.1980.
引用本文的文献
1
Purification of cowpea mosaic virus RNA replication complex: Identification of a virus-encoded 110,000-dalton polypeptide responsible for RNA chain elongation.纯化豇豆花叶病毒 RNA 复制复合体：鉴定一个病毒编码的 110000 道尔顿多肽负责 RNA 链的延伸。
Proc Natl Acad Sci U S A. 1984 Apr;81(7):1951-5. doi: 10.1073/pnas.81.7.1951.
2
Strand-specific RNA synthesis defects in a poliovirus with a mutation in protein 3A.在蛋白3A发生突变的脊髓灰质炎病毒中链特异性RNA合成缺陷
J Virol. 2003 Dec;77(23):12679-91. doi: 10.1128/jvi.77.23.12679-12691.2003.
3
Requirements for assembly of poliovirus replication complexes and negative-strand RNA synthesis.脊髓灰质炎病毒复制复合体组装及负链RNA合成的要求
J Virol. 2001 Apr;75(8):3841-50. doi: 10.1128/JVI.75.8.3841-3850.2001.
4
Replication-competent picornaviruses with complete genomic RNA 3' noncoding region deletions.具有完整基因组RNA 3'非编码区缺失的复制能力的小RNA病毒。
J Virol. 1997 Nov;71(11):8868-74. doi: 10.1128/JVI.71.11.8868-8874.1997.
5
Picornaviral structure and assembly.小核糖核酸病毒的结构与组装
Microbiol Rev. 1981 Jun;45(2):287-315. doi: 10.1128/mr.45.2.287-315.1981.
6
Production of infectious poliovirus from cloned cDNA is dramatically increased by SV40 transcription and replication signals.通过SV40转录和复制信号，从克隆的cDNA产生感染性脊髓灰质炎病毒的效率显著提高。
Nucleic Acids Res. 1984 Jun 25;12(12):5123-41. doi: 10.1093/nar/12.12.5123.
7
Antibody to a host protein prevents initiation by the poliovirus replicase.针对宿主蛋白的抗体可阻止脊髓灰质炎病毒复制酶引发反应。
J Virol. 1982 Jun;42(3):1114-7. doi: 10.1128/JVI.42.3.1114-1117.1982.
8
Characterization of a 70S polyuridylic acid polymerase isolated from foot-and-mouth disease virus-infected cells.从口蹄疫病毒感染细胞中分离出的70S多聚尿苷酸聚合酶的特性分析。
J Virol. 1981 Dec;40(3):881-9. doi: 10.1128/JVI.40.3.881-889.1981.
9
Poliovirus replication proteins: RNA sequence encoding P3-1b and the sites of proteolytic processing.脊髓灰质炎病毒复制蛋白：编码P3-1b的RNA序列及蛋白水解加工位点。
Proc Natl Acad Sci U S A. 1981 Jun;78(6):3464-8. doi: 10.1073/pnas.78.6.3464.
10
Heterogeneity of the 3' end of minus-strand RNA in the poliovirus replicative form.脊髓灰质炎病毒复制型中负链RNA 3'端的异质性
J Virol. 1980 Nov;36(2):387-94. doi: 10.1128/JVI.36.2.387-394.1980.
本文引用的文献
1
STUDIES ON EMC VIRAL RNA SYNTHESIS AND ITS LOCALIZATION IN INFECTED KREBS ASCITES CELLS.埃可病毒在感染的克雷布斯腹水癌细胞中的RNA合成及其定位研究
Virology. 1965 Jul;26:450-65. doi: 10.1016/0042-6822(65)90008-5.
2
IN VITRO SYNTHESIS OF VIRAL RNA BY THE POLIOVIRUS RNA POLYMERASE.脊髓灰质炎病毒RNA聚合酶在体外合成病毒RNA
Proc Natl Acad Sci U S A. 1964 Mar;51(3):450-6. doi: 10.1073/pnas.51.3.450.
3
A SIMPLE METHOD FOR THE QUANTITATIVE ISOLATION OF UNDEGRADED HIGH MOLECULAR WEIGHT RIBONUCLEIC ACID.一种定量分离未降解的高分子量核糖核酸的简单方法。
Biochem Biophys Res Commun. 1963 Sep 10;13:61-6. doi: 10.1016/0006-291x(63)90163-3.
4
Poliovirus-induced RNA polymerase and the effects of virus-specific inhibitors on its production.脊髓灰质炎病毒诱导的RNA聚合酶及其病毒特异性抑制剂对其产生的影响。
Proc Natl Acad Sci U S A. 1963 Jun;49(6):843-9. doi: 10.1073/pnas.49.6.843.
5
Characterization of the subunits of Q-beta replicase.Q-β复制酶亚基的表征
Nature. 1970 Nov 7;228(5271):527-33. doi: 10.1038/228527a0.
6
Subunit structure of Q-beta replicase.Q-β复制酶的亚基结构。
Nature. 1970 Nov 7;228(5271):525-7. doi: 10.1038/228525a0.
7
Localization of Q-beta maturation cistron ribosome binding site.Q-β成熟顺反子核糖体结合位点的定位
Nat New Biol. 1971 Sep 15;234(50):202-4. doi: 10.1038/newbio234202a0.
8
A comparison of the virus-specific polypeptides of encephalomyocarditis virus, human rhinovirus-1A, and poliovirus.脑心肌炎病毒、人鼻病毒1A和脊髓灰质炎病毒的病毒特异性多肽比较
Virology. 1973 Dec;56(2):439-53. doi: 10.1016/0042-6822(73)90048-2.
9
RNA polymerase complexes from mengovirus infected cells.来自感染脑心肌炎病毒的细胞的RNA聚合酶复合物。
Arch Gesamte Virusforsch. 1972;38(1):17-28. doi: 10.1007/BF01241352.
10
Proteins associated with the poliovirus RNA replication complex.与脊髓灰质炎病毒RNA复制复合体相关的蛋白质。
Virology. 1971 Nov;46(2):375-86. doi: 10.1016/0042-6822(71)90039-0.
Zotero 插件