针对宿主蛋白的抗体可阻止脊髓灰质炎病毒复制酶引发反应。
Antibody to a host protein prevents initiation by the poliovirus replicase.
作者信息
Dasgupta A, Hollingshead P, Baltimore D
出版信息
J Virol. 1982 Jun;42(3):1114-7. doi: 10.1128/JVI.42.3.1114-1117.1982.
Abstract
In vitro transcription of poliovirus RNA was catalyzed by the combination of a virus-coded polymerase and a host cell protein (host factor). Antibody to host factor inhibited template-dependent synthesis of complementary RNA where presumably RNA chain initiation occurred. On the contrary, elongation of already initiated RNA chains catalyzed by the replicase-template complex was not inhibited by anti-host factor antibody. These results strongly favored our previous notion that the host factor was needed for the initiation step of viral complementary RNA synthesis.
摘要
脊髓灰质炎病毒RNA的体外转录是由病毒编码的聚合酶和一种宿主细胞蛋白（宿主因子）共同催化的。针对宿主因子的抗体抑制了互补RNA的模板依赖性合成，而互补RNA的合成很可能发生在RNA链起始阶段。相反，由复制酶-模板复合物催化的已起始RNA链的延伸不受抗宿主因子抗体的抑制。这些结果有力地支持了我们之前的观点，即宿主因子是病毒互补RNA合成起始步骤所必需的。
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本文引用的文献
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Dependence of the activity of the poliovirus replicase on the host cell protein.脊髓灰质炎病毒复制酶活性对宿主细胞蛋白的依赖性。
Cell. 1980 Feb;19(2):423-9. doi: 10.1016/0092-8674(80)90516-4.
2
Isolation of a viral polypeptide associated with poliovirus RNA polymerase.与脊髓灰质炎病毒RNA聚合酶相关的一种病毒多肽的分离
Proc Natl Acad Sci U S A. 1974 Dec;71(12):4773-7. doi: 10.1073/pnas.71.12.4773.
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Poliovirus replicase: a soluble enzyme able to initiate copying of poliovirus RNA.脊髓灰质炎病毒复制酶：一种能够起始脊髓灰质炎病毒RNA复制的可溶性酶。
Proc Natl Acad Sci U S A. 1979 Jun;76(6):2679-83. doi: 10.1073/pnas.76.6.2679.
4
Poliovirus-specific primer-dependent RNA polymerase able to copy poly(A).能够复制聚腺苷酸（poly(A)）的脊髓灰质炎病毒特异性引物依赖性RNA聚合酶。
Proc Natl Acad Sci U S A. 1977 Sep;74(9):3677-80. doi: 10.1073/pnas.74.9.3677.
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The location of the polio genome protein in viral RNAs and its implication for RNA synthesis.脊髓灰质炎病毒基因组蛋白在病毒RNA中的定位及其对RNA合成的影响。
Nature. 1977 Jul 21;268(5617):208-13. doi: 10.1038/268208a0.
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Proc Natl Acad Sci U S A. 1977 Mar;74(3):961-5. doi: 10.1073/pnas.74.3.961.
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Virology. 1979 Jan 30;92(2):271-7. doi: 10.1016/0042-6822(79)90131-4.
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