Dormer R L, Kerbey A L, McPherson M, Manley S, Ashcroft S J, Schofield J G, Randle P H
Biochem J. 1974 May;140(2):135-42. doi: 10.1042/bj1400135.
The effects of Ni(2+) on the release of amylase from rat parotids, insulin from mouse pancreatic islets and growth hormone from bovine pituitary slices were investigated. In all these secretory systems, Ni(2+) was shown to inhibit release evoked by a variety of stimuli both physiological and pharmacological. Measurements of rates of substrate oxidation and tissue concentrations of ATP and 3':5'-cyclic AMP suggest that this inhibitory action of Ni(2+) does not arise through an effect on energy metabolism or cyclic AMP metabolism. It is concluded that although some effects of Ni(2+) may involve antagonism between Ni(2+) and Ca(2+) in stimulus-secretion coupling, others appear to be independent of Ca(2+). It is suggested that Ni(2+) may block exocytosis by interfering with either secretory-granule migration or membrane fusion and microvillus formation. The possible mode of action of Ni(2+) and its potential use as a tool in the study of exocytosis are discussed.
研究了镍离子（Ni(2+)）对大鼠腮腺淀粉酶释放、小鼠胰岛胰岛素释放以及牛垂体切片生长激素释放的影响。在所有这些分泌系统中，Ni(2+)均显示出抑制由多种生理和药理刺激所诱发的释放。底物氧化速率以及ATP和3':5'-环磷酸腺苷（3':5'-cyclic AMP）的组织浓度测量结果表明，Ni(2+)的这种抑制作用并非通过对能量代谢或环磷酸腺苷代谢的影响而产生。得出的结论是，尽管Ni(2+)的某些作用可能涉及在刺激-分泌偶联过程中Ni(2+)与钙离子（Ca(2+)）之间的拮抗作用，但其他作用似乎与Ca(2+)无关。有人提出，Ni(2+)可能通过干扰分泌颗粒迁移或膜融合以及微绒毛形成来阻断胞吐作用。文中讨论了Ni(2+)可能的作用方式及其作为研究胞吐作用工具的潜在用途。
