植物乳杆菌一种新的烟酰胺腺嘌呤二核苷酸依赖性氢芳香脱氢酶及其在（-）-t-3，t-4-二羟基环己烷-c-1-羧酸盐形成中的作用。
A new nicotinamide-adenine dinucleotide-dependent hydroaromatic dehydrogenase of Lactobacillus plantarum and its role in formation of (minus)t-3,t-4-dihydroxycyclohexane-c-1-carboxylate.
作者信息
Whiting G C, Coggins R A
出版信息
Biochem J. 1974 Jul;141(1):35-42. doi: 10.1042/bj1410035.
Abstract
- A new induced NAD-dependent hydroaromatic dehydrogenase was isolated from a cell-free extract of Lactobacillus plantarum 13a and purified 175-fold. 2. The enzyme catalyses the oxidation of (-)-quinate, (-)-shikimate, (-)-dihydroshikimate and (-)t-3,t-4-dihydroxycyclohexane-c-1-carboxylate with NAD(+), and the reverse action with NADH. 3. The K(m) values at the optimal pH10.0 for these substrates are 0.85, 0.75, 0.52 and 0.74mm respectively, and the corresponding values for NAD(+) are 0.45, 0.26, 0.34 and 0.36mm respectively. 4. The stereochemical requirements of the enzyme and the role it may play in the reduction of (-)-quinate to (-)t-3,t-4-dihydroxycyclohexane-c-1-carboxylate are discussed and a pathway is suggested.
摘要
- 从植物乳杆菌13a的无细胞提取物中分离出一种新的诱导型NAD依赖性氢化芳香脱氢酶，并将其纯化了175倍。2. 该酶催化(-)-奎尼酸、(-)-莽草酸、(-)-二氢莽草酸和(-)-t-3,t-4-二羟基环己烷-c-1-羧酸盐与NAD(+)的氧化反应，以及与NADH的逆反应。3. 在最佳pH10.0时，这些底物的K(m)值分别为0.85、0.75、0.52和0.74mm，NAD(+)的相应值分别为0.45、0.26、0.34和0.36mm。4. 讨论了该酶的立体化学要求及其在将(-)-奎尼酸还原为(-)-t-3,t-4-二羟基环己烷-c-1-羧酸盐中可能起的作用，并提出了一条途径。
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