Herman P G, Utsunomiya R, Hessel S J
Immunology. 1979 Apr;36(4):793-7.
We have assessed the effects of antigenic stimulation on the regional blood flow of the lymph node. In seventeen New Zealand white rabbits, the regional blood flow to the popliteal lymph nodes was determined using 9 and 15 micron microspheres at 16, 40, and 144 h following antigen administration. The regional blood flow in the resting lymph node measured with 15 micron spheres was 0 . 46 +/- 0 . 07 ml g-1 min-1, with 9 micron spheres 0 . 26 +/- 0 . 03 ml g-1 min-1. There was a significant 'differential flow' (the difference in blood flow between the experimental and control nodes) measured with 15 micron spheres (P less than 0 . 05). This increase in blood flow was larger early (16 h) than late (144 h) following antigen administration (P less than 0 . 01). There was significant (P less than 0 . 05) 'shunt flow' (the difference in blood flow measured with 15 micron and 9 micron spheres) in the lymph node, with more shunting early (16 h) than late (144 h) following antigen administration. No significant shunting was seen in the kidney or thigh muscle. Thus, we have established: (1) a significant increase in blood flow following antigenic stimulus; (2) shunting of 9 micron microspheres in the resting popliteal lymph node; (3) increased shunting following antigenic stimulus; and (4) that the increase in nodal blood flow was, to a large extent, secondary to increased shunt flow. Both the increased blood flow and the shunting shortly after antigenic stimulus may be important in the evolution of the immune response.
我们评估了抗原刺激对淋巴结局部血流的影响。在17只新西兰白兔中，于抗原注射后16、40和144小时，使用9微米和15微米的微球测定腘窝淋巴结的局部血流。用15微米微球测得的静息淋巴结局部血流为0.46±0.07毫升·克⁻¹·分钟⁻¹，用9微米微球测得为0.26±0.03毫升·克⁻¹·分钟⁻¹。用15微米微球测得存在显著的“差异血流”（实验组和对照组淋巴结之间的血流差异）（P<0.05）。抗原注射后早期（16小时）的血流增加幅度大于晚期（144小时）（P<0.01）。淋巴结中存在显著的（P<0.05）“分流”（用15微米和9微米微球测得的血流差异），抗原注射后早期（16小时）的分流比晚期（144小时）更多。在肾脏或大腿肌肉中未见明显分流。因此，我们确定了：（1）抗原刺激后血流显著增加；（2）静息腘窝淋巴结中有9微米微球的分流；（3）抗原刺激后分流增加；（4）淋巴结血流增加在很大程度上继发于分流增加。抗原刺激后不久的血流增加和分流可能在免疫反应的演变中起重要作用。
