Tilgen W
J Cutan Pathol. 1979 Feb;6(1):18-30. doi: 10.1111/j.1600-0560.1979.tb00302.x.
Biopsies from typical lesions of pitted keratolysis from five patients have been investigated by electron microscopy to provide information on colonization and morphology of the microorganisms involved in this disease. A part of the biopsies was inoculated in culture media, and Corynebacteria were selected for further examination. Electron microscopy revealed a great variability in the morphologic feature of bacteria concerning size, shape, capsule, cell wall, cross wall formation, plasma membrane, nucleoplasm, cytoplasmic organelles and constituents including mesosomes, ribosomes, volutin granules, and glycogen particles. The pleomorphism is thought to be due in part to the diversity of preparation techniques used in this study and in part to different growth conditions in vivo and in vitro. As a consequence the present findings are only partly comparable with previously published data on bacteria involved in skin diseases. Evidence is presented that bacteria inducing pitted keratolysis may be able to destroy keratin by means of hydrolytic enzymatic activity.
对5例凹坑状角质松解症典型皮损的活检标本进行了电子显微镜检查，以获取有关该疾病相关微生物定植和形态的信息。将部分活检标本接种于培养基中，选择棒状杆菌作进一步检查。电子显微镜检查显示，细菌在大小、形状、荚膜、细胞壁、横壁形成、质膜、核质、细胞质细胞器及成分（包括中介体、核糖体、异染颗粒和糖原颗粒）等形态特征上存在很大差异。这种多形性被认为部分归因于本研究中使用的制备技术的多样性，部分归因于体内和体外不同的生长条件。因此，目前的研究结果仅部分与先前发表的有关皮肤疾病相关细菌的数据可比。有证据表明，引起凹坑状角质松解症的细菌可能能够通过水解酶活性破坏角蛋白。
