Argyris B F, Askonas B A
Immunology. 1968 Mar;14(3):379-92.
The functional activities of peritoneal cells in exudates of normal mice were studied. Antigen-containing peritoneal cells were tested for their ability to induce antibody by transfer into normal syngeneic recipients. Collection of peritoneal cells at varying time intervals following intraperitoneal injection of formalin-killed pneumococci or capsular polysaccharide, resulted in a decrease with time in the ability of these cells to induce antibody in the recipient mice. This finding could be ascribed to changes in the cell population in the peritoneum as a result of antigen administration rather than to loss of immunogenic material from these cells. Evidence for this comes from experiments in which peritoneal cells collected ½-hour after antigen administration were maintained for up to 24 hours. The immunogenicity of carbohydrate antigen in these cells persists for at least 24 hours. Furthermore our morphological observations showed a drop in macrophages following antigen administration. Our findings indicate that peritoneal exudates also contain immunocompetent cells. For this study we used recipient mice irradiated with 650–800 r, which completely suppressed their antibody response. Peritoneal cells collected 2 hours after injection of formalin-killed pneumococci led to serum antibody in the irradiated recipients 7 days later. By partially fractionating peritoneal cells on glass surfaces, it could be shown that the cells not adhering to glass (lymphocytes and medium sized cells) were mainly responsible for this antibody response. Cells adhering to glass which contained most of the antigen-containing large macrophages and also medium sized cells, were much less effective in inducing antibody. In addition, peritoneal cells induced with thioglycollate medium consisting mainly of large macrophages and very few lymphocytes, actively induced antibody in normal recipients but failed to restore antibody production in the highly irradiated mice. The transfer of antibody formation to irradiated mice thus is due to immunocompetent cells in normal peritoneal exudates.
对正常小鼠渗出液中腹膜细胞的功能活性进行了研究。将含抗原的腹膜细胞转移至同基因正常受体小鼠体内，检测其诱导抗体产生的能力。在腹腔注射福尔马林灭活肺炎球菌或荚膜多糖后的不同时间间隔收集腹膜细胞，结果发现这些细胞诱导受体小鼠产生抗体的能力随时间下降。这一发现可归因于抗原注入后腹膜中细胞群体的变化，而非这些细胞免疫原性物质的丧失。这一结论的证据来自于抗原注入半小时后收集的腹膜细胞可维持长达24小时的实验。这些细胞中碳水化合物抗原的免疫原性至少持续24小时。此外，我们的形态学观察显示抗原注入后巨噬细胞数量减少。我们的研究结果表明，腹膜渗出液中也含有免疫活性细胞。在本研究中，我们使用了接受650 - 800伦琴辐射的受体小鼠，这完全抑制了它们的抗体反应。注射福尔马林灭活肺炎球菌2小时后收集的腹膜细胞，7天后可使受辐射受体小鼠产生血清抗体。通过在玻璃表面对腹膜细胞进行部分分离，可以发现不粘附于玻璃的细胞（淋巴细胞和中等大小细胞）是产生这种抗体反应的主要原因。粘附于玻璃的细胞含有大多数含抗原的大巨噬细胞以及中等大小细胞，在诱导抗体方面效果要差得多。此外，用主要由大巨噬细胞和极少数淋巴细胞组成的巯基乙酸盐培养基诱导的腹膜细胞，能在正常受体小鼠中积极诱导抗体产生，但无法恢复高度辐射小鼠的抗体产生。因此，向辐射小鼠转移抗体形成是由于正常腹膜渗出液中的免疫活性细胞。
