Inchley C J
Clin Exp Immunol. 1969 Jul;5(1):173-87.
The response of macrophages from the livers and spleens of mice given a single immunizing dose of T4 bacteriophage has been studied. Following their rapid removal from the circulation, phage particles were found to be concentrated in the liver to a level twelve times that for the spleen. Investigation of the fate of ingested phage showed that it was disposed of more rapidly in the liver than in the spleen, as measured by the disappearance of viable T4 particles and by the loss of radioactive label following injection of [I]T4. It was also found that antigen-containing Kupffer cells could elicit little or no antibody synthesis on transfer into normal syngeneic recipients, or on incubation with lymphoid cells . It is suggested that these macrophages differ from other components of the reticulo-endothelial system in their treatment of T4 antigen, and may be concerned mainly with its breakdown and disposal rather than with providing a stimulus for the initiation of antibody synthesis.
对单次接种免疫剂量T4噬菌体的小鼠肝脏和脾脏中的巨噬细胞反应进行了研究。噬菌体颗粒从循环中被快速清除后，发现其在肝脏中的浓度是脾脏中的12倍。对摄入噬菌体的命运的研究表明，通过存活T4颗粒的消失以及注射[I]T4后放射性标记的损失来衡量，噬菌体在肝脏中的清除速度比在脾脏中更快。还发现，含抗原的库普弗细胞在转移到同基因正常受体中或与淋巴细胞一起孵育时，几乎不会引发抗体合成。有人提出，这些巨噬细胞在处理T4抗原方面与网状内皮系统的其他成分不同，可能主要参与其分解和清除，而不是为抗体合成的启动提供刺激。
