The rapid and large scale separation of peritoneal macrophages.

A simple method for rapid and large scale (over 10 cells) separation of peritoneal macrophages by incubation in a glass-bead column and elution with buffered ethylenediamine tetraacetic acid (EDTA) solution is described. Macrophages obtained were about 90 per cent pure with the recovery rates of 38–53 per cent depending upon the sample size, and 83 per cent viability. Lymphocyte contamination was reduced to 5 per cent or less.