Taranta A, Cuppari G, Quagliata F
J Exp Med. 1969 Apr 1;129(4):605-22. doi: 10.1084/jem.129.4.605.
The ability of streptolysin S preparations to induce high percentages of transformation in human peripheral blood lymphocytes was confirmed in a series of apparently healthy donors. Transforming activity was not demonstrated in the two media used for streptolysin S production, nor in control preparations in which a strain each of Streptococcus viridans, Staphylococcus aureus (nonhemolytic), and Diplococcus pneumoniae was substituted for the beta hemolytic streptococcal strain used for streptolysin S production. The relation of the hemolytic activity to the lymphocyte transforming activity of streptolysin S preparations was studied by means of inactivation and fractionation experiments. Heating produced a loss in both activities, but more in the hemolytic than in the transforming activity. The transformation obtained with a heated preparation had a high degree of correlation with that obtained with the unheated preparation in a series of normal subjects and patients with various rheumatic diseases, whose lymphocytes were often less responsive to stimulation with streptolysin S preparations (both heated and unheated) than the lymphocytes of the normal subjects studied. Treatment of streptolysin S preparations with chymotrypsin, vegetable lecithin, or trypan blue (the latter in minute amounts) resulted in preparations with no detectable hemolytic activity but with undiminished lymphocyte transforming activity. Chromatographic fractionations on DEAE-Sephadex columns yielded fractions endowed with transforming but not with hemolytic activity, and other fractions endowed with hemolytic but not with transforming activity. The recovery of the hemolytic activity was not complete and quantitation of the recovery of the transforming activity was not attempted. These experiments indicate that the hemolytic and transforming activities of streptolysin S preparations are independent of each other, and specifically that they are the attributes of two different streptococcal products, one of which is streptolysin S. The other is a nonhemolytic streptococcal product present in streptolysin S preparations but previously unrecognized. Some implications of these findings are discussed.
在一系列看似健康的供体中，证实了链球菌溶血素S制剂能够在人外周血淋巴细胞中诱导高比例的转化。在用于生产链球菌溶血素S的两种培养基中，以及在对照制剂中均未显示出转化活性，在对照制剂中，分别用一株草绿色链球菌、金黄色葡萄球菌（非溶血性）和肺炎双球菌替代用于生产链球菌溶血素S的β溶血性链球菌菌株。通过灭活和分级分离实验研究了链球菌溶血素S制剂的溶血活性与淋巴细胞转化活性之间的关系。加热会使两种活性都丧失，但溶血活性丧失得更多。在一系列正常受试者和患有各种风湿性疾病的患者中，用加热制剂获得的转化与用未加热制剂获得的转化具有高度相关性，这些患者的淋巴细胞对链球菌溶血素S制剂（加热和未加热）刺激的反应通常比所研究的正常受试者的淋巴细胞更弱。用胰凝乳蛋白酶、植物卵磷脂或台盼蓝（微量）处理链球菌溶血素S制剂，得到的制剂没有可检测到的溶血活性，但淋巴细胞转化活性未降低。在DEAE-葡聚糖凝胶柱上进行色谱分级分离，得到具有转化活性但无溶血活性的级分，以及具有溶血活性但无转化活性的其他级分。溶血活性的回收率不完全，未尝试对转化活性的回收率进行定量。这些实验表明，链球菌溶血素S制剂的溶血活性和转化活性彼此独立，具体而言，它们是两种不同的链球菌产物的特性，其中一种是链球菌溶血素S。另一种是存在于链球菌溶血素S制剂中但以前未被识别的非溶血性链球菌产物。讨论了这些发现的一些意义。
