人类红细胞中谷胱甘肽代谢的酶学研究。
Studies in the enzymology of glutathione metabolism in human erythrocytes.
作者信息
Jackson R C
出版信息
Biochem J. 1969 Feb;111(3):309-15. doi: 10.1042/bj1110309.
Abstract
Spectrophotometric assay methods are described for glutathione synthetase, gamma-glutamylcysteine synthetase and gamma-glutamyl transpeptidase of erythrocytes. The contents of these enzymes in normal human erythrocytes are reported. Erythrocyte glutathione synthetase is inhibited by ADP; this inhibition is competitive with respect to ATP. gamma-Glutamylcysteine synthetase is subject to feedback inhibition by GSH, and is also inhibited by NADH, and to a lesser extent by NAD(+) and NADPH. This enzyme is irreversibly inactivated by cysteamine.
摘要
本文描述了用于检测红细胞中谷胱甘肽合成酶、γ-谷氨酰半胱氨酸合成酶和γ-谷氨酰转肽酶的分光光度测定方法。报告了这些酶在正常人红细胞中的含量。红细胞谷胱甘肽合成酶受ADP抑制；这种抑制作用对ATP而言是竞争性的。γ-谷氨酰半胱氨酸合成酶受到GSH的反馈抑制，也受到NADH的抑制，并且在较小程度上受到NAD(+)和NADPH的抑制。该酶被半胱胺不可逆地灭活。
相似文献
1
Studies in the enzymology of glutathione metabolism in human erythrocytes.人类红细胞中谷胱甘肽代谢的酶学研究。
Biochem J. 1969 Feb;111(3):309-15. doi: 10.1042/bj1110309.
2
Effects of adenosine phosphates and nicotinamide nucleotides on pyruvate carboxylase from baker's yeast.三磷酸腺苷和烟酰胺核苷酸对面包酵母丙酮酸羧化酶的影响。
Biochem J. 1969 May;112(5):755-62. doi: 10.1042/bj1120755.
3
Reduction of arsenate to arsenite by human erythrocyte lysate and rat liver cytosol - characterization of a glutathione- and NAD-dependent arsenate reduction linked to glycolysis.人红细胞裂解物和大鼠肝细胞溶胶将砷酸盐还原为亚砷酸盐——与糖酵解相关的谷胱甘肽和NAD依赖性砷酸盐还原的特性
Toxicol Sci. 2005 Jun;85(2):847-58. doi: 10.1093/toxsci/kfi157. Epub 2005 Mar 23.
4
Essential arginine residues in the pyridine nucleotide binding sites of glutathione reductase.谷胱甘肽还原酶吡啶核苷酸结合位点中的必需精氨酸残基。
Biochim Biophys Acta. 1982 Feb 4;701(1):119-26. doi: 10.1016/0167-4838(82)90319-3.
5
6
Patients with genetic defects in the gamma-glutamyl cycle.γ-谷氨酰循环存在基因缺陷的患者。
Chem Biol Interact. 1998 Apr 24;111-112:113-21. doi: 10.1016/s0009-2797(97)00155-5.
7
CHROMATE INDUCED INHIBITION OF GLUTATHIONE REDUCTASE (GSSG-R) IN THE HUMAN ERYTHROCYTE.
Trans Assoc Am Physicians. 1964;77:88-99.
8
Bovine lens gamma-glutamylcysteine synthetase. Inhibition by glutathione and adenine nucleotides.
Eur J Biochem. 1983 Jul 1;133(3):635-40. doi: 10.1111/j.1432-1033.1983.tb07510.x.
9
Glutathione biosynthesis in human erythrocytes. I. Identification of the enzymes of glutathione synthesis in hemolysates.人类红细胞中的谷胱甘肽生物合成。I. 溶血产物中谷胱甘肽合成酶的鉴定。
J Clin Invest. 1971 Mar;50(3):507-13. doi: 10.1172/JCI106519.
10
Effect of pyridine nucleotides on ATP synthesis and hydrolysis by the mitochondrial ATPase.吡啶核苷酸对线粒体ATP酶合成及水解ATP的影响。
Biochem Int. 1990 Nov;22(4):677-84.
引用本文的文献
1
λ-Glutamylcysteine synthetase in higher plants: catalytic properties and subcellular localization.高等植物中的λ-谷氨酰半胱氨酸合成酶：催化特性和亚细胞定位。
Planta. 1990 Mar;180(4):603-12. doi: 10.1007/BF02411460.
2
Glutathione synthesis and turnover in the human erythrocyte: alignment of a model based on detailed enzyme kinetics with experimental data.人红细胞中谷胱甘肽的合成和转化：基于详细酶动力学模型与实验数据的整合。
J Biol Chem. 2010 Jul 30;285(31):23557-67. doi: 10.1074/jbc.M109.067017. Epub 2010 May 24.
3
Mechanisms of gamma-glutamylcysteine ligase regulation.γ-谷氨酰半胱氨酸连接酶的调控机制。
Biochim Biophys Acta. 2006 Feb;1760(2):233-44. doi: 10.1016/j.bbagen.2005.10.010. Epub 2005 Nov 17.
4
Some effects of chlorambucil on enzymes of glutathione metabolism in drug-sensitive and -resistant strains of the Yoshida ascites sarcoma.苯丁酸氮芥对吉田腹水肉瘤药物敏感株和耐药株谷胱甘肽代谢酶的一些影响。
Biochem J. 1969 Feb;111(4):603-6. doi: 10.1042/bj1110603.
5
Glutathione biosynthesis in human erythrocytes. I. Identification of the enzymes of glutathione synthesis in hemolysates.人类红细胞中的谷胱甘肽生物合成。I. 溶血产物中谷胱甘肽合成酶的鉴定。
J Clin Invest. 1971 Mar;50(3):507-13. doi: 10.1172/JCI106519.
6
[Glutathione peroxidase: enzymology and biological aspects].[谷胱甘肽过氧化物酶：酶学与生物学方面]
Klin Wochenschr. 1971 Jun 15;49(12):669-83. doi: 10.1007/BF01487101.
7
Assay, purification, properties and mechanism of action of gamma-glutamylcysteine synthetase from the liver of the rat and Xenopus laevis.大鼠和非洲爪蟾肝脏中γ-谷氨酰半胱氨酸合成酶的测定、纯化、性质及作用机制
Biochem J. 1973 Aug;133(4):667-78. doi: 10.1042/bj1330667.
8
Formation of 5-oxoproline from glutathione in erythrocytes by the gamma-glutamyltranspeptidase-cyclotransferase pathway.通过γ-谷氨酰转肽酶-环转移酶途径，红细胞中的谷胱甘肽形成5-氧代脯氨酸。
Proc Natl Acad Sci U S A. 1974 Feb;71(2):293-7. doi: 10.1073/pnas.71.2.293.
9
Effect of Methylene Blue on glutamate and reduced glutathione of rabbit erythrocytes.亚甲蓝对兔红细胞中谷氨酸和还原型谷胱甘肽的影响。
Biochem J. 1977 Dec 15;168(3):587-9. doi: 10.1042/bj1680587.
本文引用的文献
1
Biosynthesis of glutathione by rat erythrocytes.大鼠红细胞中谷胱甘肽的生物合成。
Biochem J. 1959 Jul;72(3):389-95. doi: 10.1042/bj0720389.
2
Determination of glutathione.谷胱甘肽的测定
Methods Biochem Anal. 1955;2:259-78. doi: 10.1002/9780470110188.ch9.
3
Enzymatic synthesis of gamma-glutamylcysteine.γ-谷氨酰半胱氨酸的酶促合成
J Biol Chem. 1955 Jun;214(2):639-46.
4
The metabolic behavior of reduced glutathione in human and avian erythrocytes.还原型谷胱甘肽在人和禽类红细胞中的代谢行为。
J Biol Chem. 1955 Apr;213(2):769-76.
5
SOME BIOCHEMICAL ASPECTS OF LEUKAEMIAS: THE APPEARANCE OF A SOLUBLE DISULPHIDE IN THE BLOOD IN CHRONIC GRANULOCYTIC LEUKAEMIA.白血病的一些生化方面：慢性粒细胞白血病患者血液中可溶性二硫化物的出现。
Br J Cancer. 1964 Dec;18(4):809-17. doi: 10.1038/bjc.1964.94.
6
Studies on normal and leukemic leukocytes. III. Pyridine nucleotides.正常及白血病白细胞的研究。III. 吡啶核苷酸。
J Clin Invest. 1962 Feb;41(2):230-4. doi: 10.1172/JCI104474.
7
The reduction of disulphides by human erythrocytes.人类红细胞对二硫化物的还原作用。
Biochem J. 1962 Jan;82(1):192-7. doi: 10.1042/bj0820192.
8
Acidic peptides of the lens. 6. Metabolism of gamma-glutamyl peptides in subcellular fractions of rabbit liver.晶状体的酸性肽。6. 兔肝脏亚细胞组分中γ-谷氨酰肽的代谢
Biochem J. 1961 Apr;79(1):118-28. doi: 10.1042/bj0790118.
9
10
Estimation and identification of thiols in rat spleen after cysteine or glutathione treatment: relevance to protection against nitrogen mustards.半胱氨酸或谷胱甘肽处理后大鼠脾脏中硫醇的估计与鉴定：与氮芥防护的相关性
Biochem Pharmacol. 1966 Jul;15(7):809-16. doi: 10.1016/0006-2952(66)90157-2.
Zotero 插件