Dajani A S, Wannamaker L W
J Bacteriol. 1969 Mar;97(3):985-91. doi: 10.1128/jb.97.3.985-991.1969.
Staphylococcal skin isolates belonging to phage type 71 were found to produce a bactericidal substance against some streptococci, pneumococci, and corynebacteria. Fifteen strains of group A streptococci belonging to 13 different M types, group C streptococci, and group D streptococci were uniformly inhibited on solid media and in broth by membrane-filtered supernatant fluids of the staphylococcal broth cultures. Inhibition of group G streptococci and other staphyloccoci was variable, and no inhibition of group B streptococci or of a variety of gram-negative rods was demonstrable. A quantitative variation observed to exist among susceptible organisms was a function of the inoculum size of the inhibited strains. The bactericidal substance could be detected best from 24 to 48 hr after inoculation of the staphylococci in tryptic soy broth or in a dialysate of tryptic soy broth. Little or no bactericidal activity was noted when the organisms were grown in several other liquid media. The bactericidal substance was nondialyzable and could be precipitated with ammonium sulfate. It was heat-stable and its activity was not altered within a pH range of 4.0 to 8.5. Pronase and three times crystallized trypsin totally abolished its activity. The concentrated ammonium sulfate precipitate could be fractionated on a Sephadex G-100 column into several peaks, with the bactericidal activity localized to a single peak.
发现属于噬菌体71型的葡萄球菌皮肤分离株可产生一种针对某些链球菌、肺炎球菌和棒状杆菌的杀菌物质。15株属于13种不同M型的A组链球菌、C组链球菌和D组链球菌，在固体培养基和肉汤中均被葡萄球菌肉汤培养物的膜过滤上清液均匀抑制。G组链球菌和其他葡萄球菌的抑制情况各不相同，未发现对B组链球菌或多种革兰氏阴性杆菌有抑制作用。在敏感菌中观察到的定量差异是被抑制菌株接种量的函数。接种葡萄球菌于胰蛋白酶大豆肉汤或胰蛋白酶大豆肉汤透析液中24至48小时后，最易检测到杀菌物质。当在其他几种液体培养基中培养这些微生物时，几乎没有或没有杀菌活性。该杀菌物质不能透析，可被硫酸铵沉淀。它对热稳定，在pH值4.0至8.5范围内其活性不变。链霉蛋白酶和三次结晶的胰蛋白酶可完全消除其活性。硫酸铵浓缩沉淀物可在Sephadex G - 100柱上分离成几个峰，杀菌活性集中在一个单一峰上。
