Lichtenstein L M, Gewurz H, Adkinson N F, Shin H S, Mergenhagen S E
Immunology. 1969 Mar;16(3):327-36.
The incubation of endotoxin derived from or with fresh guinea-pig serum leads to the production of a factor which contracts guinea-pig ileum. This factor has been identified as an anaphylatoxin since it causes tachyphylaxis and its activity is abolished by antihistamines. The activity is not generated if the serum has been heat inactivated or if the reaction is carried out at 0° or in the presence of EDTA. The complement system (C′) is implicated in the generation of this anaphylatoxin by the above data and by static and kinetic C′ consumption studies which show a correlation between the disappearance of the late acting C′ and the appearance of gut contracting activity. Endotoxoid preparations which do not utilize C′ are not able to generate anaphylatoxin. A further parallel between classical anaphylatoxin and the endotoxin-generated factor is found in the pattern of mammalian sera which support the reaction. In view of the similarities between the biological properties of anaphylatoxin and the syndrome of endotoxic shock, it is suggested that some of the manifestations of the latter might result from an interaction between endotoxin and the C′ system leading to the generation of an anaphylatoxin.
源自[具体来源1]或[具体来源2]的内毒素与新鲜豚鼠血清一起孵育会产生一种使豚鼠回肠收缩的因子。这种因子已被鉴定为过敏毒素，因为它会引起快速耐受性，并且其活性会被抗组胺药消除。如果血清已被热灭活，或者反应在0°下进行或在乙二胺四乙酸（EDTA）存在的情况下进行，则不会产生该活性。根据上述数据以及补体系统（C′）的静态和动力学消耗研究（这些研究表明晚期作用的C′的消失与肠道收缩活性的出现之间存在相关性），补体系统（C′）与这种过敏毒素的产生有关。不利用C′的类毒素制剂无法产生过敏毒素。在支持该反应的哺乳动物血清模式中发现了经典过敏毒素与内毒素产生的因子之间的进一步相似之处。鉴于过敏毒素的生物学特性与内毒素休克综合征之间的相似性，有人提出后者的某些表现可能是由于内毒素与C′系统之间的相互作用导致过敏毒素的产生。
