Vogt M T, Thomas C, Vassallo C L, Basford R E, Gee J B
J Clin Invest. 1971 Feb;50(2):401-10. doi: 10.1172/JCI106507.
Phagocytosis by rabbit alveolar macrophages (AM) is accompanied by increases in O(2) consumption, glucose oxidation, and H(2)O(2) formation. Two aspects of the interrelations between these metabolic features of phagocytosis have been studied.First, the following evidence indicates that glutathione, glutathione reductase, and peroxidase serve as a cytoplasmic shuttle between H(2)O(2) and NADPH-dependent glucose oxidation: (a) AM contain 5.9 mmumoles of reduced glutathione per 10(6) cells and exhibit glutathione peroxidase and NADPH-specific glutathione reductase activity; (b) oxidized glutathione potentiates NADP stimulation of glucose oxidation; (c) an artificial H(2)O(2)-generating system stimulates glucose oxidation; (d) the cell penetrating thiol inhibitor, N-ethylmaleimide diminishes glucose oxidation. This effect largely depends on inhibition of the glutathione system rather than on inhibition of either H(2)O(2) formation or enzymes directly subserving glucose oxidation.Second, three potential H(2)O(2)-generating oxidases have been sought. No cyanide-insensitive NADH or NADPH oxidase activity could be detected. D-amino acid oxidase activity was 0.48 +/-0.07 U/10(6) cells with D-alanine as substrate.
兔肺泡巨噬细胞（AM）的吞噬作用伴随着耗氧量、葡萄糖氧化和过氧化氢生成的增加。已经研究了吞噬作用这些代谢特征之间相互关系的两个方面。首先，以下证据表明谷胱甘肽、谷胱甘肽还原酶和过氧化物酶作为过氧化氢与NADPH依赖性葡萄糖氧化之间的细胞质穿梭体：（a）每10^6个细胞中，AM含有5.9微摩尔还原型谷胱甘肽，并表现出谷胱甘肽过氧化物酶和NADPH特异性谷胱甘肽还原酶活性；（b）氧化型谷胱甘肽增强NADP对葡萄糖氧化的刺激作用；（c）人工过氧化氢生成系统刺激葡萄糖氧化；（d）细胞穿透性硫醇抑制剂N-乙基马来酰亚胺减少葡萄糖氧化。这种作用很大程度上取决于对谷胱甘肽系统的抑制，而不是对过氧化氢生成或直接参与葡萄糖氧化的酶的抑制。其次，已寻找三种潜在的过氧化氢生成氧化酶。未检测到对氰化物不敏感的NADH或NADPH氧化酶活性。以D-丙氨酸为底物时，D-氨基酸氧化酶活性为0.48±0.07 U/10^6个细胞。
