吞噬作用期间肺泡巨噬细胞中过氧化氢酶依赖性过氧化代谢
Catalase-dependent peroxidative metabolism in the alveolar macrophage during phagocytosis.
作者信息
Gee J B, Vassallo C L, Bell P, Kaskin J, Basford R E, Field J B
出版信息
J Clin Invest. 1970 Jun;49(6):1280-7. doi: 10.1172/JCI106340.
EVIDENCE FOR THE PRESENCE OF PEROXIDATIVE METABOLISM IN RABBIT ALVEOLAR MACROPHAGES (AM) HAS BEEN OBTAINED FROM THE FOLLOWING OBSERVATIONS: (a) catalase is present in high concentrations; (b) peroxidase activity could not be detected employing guaiacol as substrate; (c) the irreversible inhibition of AM catalase by aminotriazole served as a detection system for H(2)O(2) and demonstrated increased intracellular H(2)O(2) after phagocytosis; (d) formate oxidation, a marker of catalase-dependent peroxidations, occurs in resting AM and is increased by phagocytosis; (c) measurements of H(2)O(2) accumulation in a dialysate of AM demonstrated twofold increase during phagocytosis; and (f) aminotriazole diminishes O(2) utilization and (14)CO(2) production from labelled glucose and pyruvate. It is concluded that, while catalase-dependent H(2)O(2) metabolism is not essential for particle entry, this pathway represents one of the metabolic pathways stimulated by particle entry in the AM.
从以下观察结果中获得了兔肺泡巨噬细胞（AM）中存在过氧化代谢的证据：（a）过氧化氢酶以高浓度存在；（b）以愈创木酚为底物未检测到过氧化物酶活性；（c）氨基三唑对AM过氧化氢酶的不可逆抑制用作H₂O₂的检测系统，并证明吞噬后细胞内H₂O₂增加；（d）甲酸氧化是过氧化氢酶依赖性过氧化的标志物，在静息AM中发生，并因吞噬而增加；（e）对AM透析液中H₂O₂积累的测量表明吞噬过程中增加了两倍；以及（f）氨基三唑减少了标记葡萄糖和丙酮酸的O₂利用和¹⁴CO₂产生。得出的结论是，虽然过氧化氢酶依赖性H₂O₂代谢对于颗粒进入不是必需的，但该途径是AM中颗粒进入刺激的代谢途径之一。
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