Myers W F, Osterman J V, Wisseman C L
J Bacteriol. 1972 Jan;109(1):89-95. doi: 10.1128/jb.109.1.89-95.1972.
Rickettsia quintana grew in a liquid medium consisting of a brain-heart infusion base supplemented with starch and hematin. The growth requirement for hematin could not be substituted by compounds of known catalytic activity for H(2)O(2), viz., catalase, potassium pyruvate, or charcoal, or by the reducing compounds sodium sulfite and sodium thioglycollate. R. quintana was catalase-negative, but no H(2)O(2) production could be demonstrated by the catalase-aminotriazole technique. A minimum inoculum giving 10(5) cells/ml was required to initiate growth. The generation time at 33 C was 10 hr. The temperature range for growth was 28 to 37 C. Growth was enhanced when succinate or glutamate was added as energy source.
五日热立克次体在一种液体培养基中生长，该培养基由补充了淀粉和血红素的脑心浸液基础培养基组成。血红素的生长需求不能被对H₂O₂具有已知催化活性的化合物替代，即过氧化氢酶、丙酮酸钾或木炭，也不能被还原化合物亚硫酸钠和巯基乙酸钠替代。五日热立克次体过氧化氢酶阴性，但通过过氧化氢酶 - 氨基三唑技术未检测到H₂O₂产生。启动生长所需的最小接种量为每毫升10⁵个细胞。33℃时的代时为10小时。生长温度范围为28至37℃。添加琥珀酸盐或谷氨酸盐作为能源时生长增强。
