Kemp D J, Dyer P Y, Rogers G E
J Cell Biol. 1974 Jul;62(1):114-31. doi: 10.1083/jcb.62.1.114.
The synthesis of keratin proteins during development of the embryonic chick feather was studied by quantitative gel electrophoresis of the reduced and carboxymethylated proteins. The results demonstrated a coordinated synthesis of the major keratin proteins, during and after the onset of keratin synthesis. The results from gel electrophoresis correlated well with electron microscope visualization or keratin fibrils in the developing feathers. Autoradiography at the electron microscope level indicated that the feather cells lose the ability to synthesize DNA before keratin synthesis begins, but retain the ability to synthesize RNA after keratin synthesis begins.
通过对还原和羧甲基化蛋白质进行定量凝胶电泳，研究了胚胎期雏鸡羽毛发育过程中角蛋白的合成。结果表明，在角蛋白合成开始期间及之后，主要角蛋白是协同合成的。凝胶电泳结果与发育中羽毛角蛋白纤维的电子显微镜观察结果高度相关。电子显微镜水平的放射自显影表明，羽毛细胞在角蛋白合成开始前失去合成DNA的能力，但在角蛋白合成开始后仍保留合成RNA的能力。
