编码鸡胚胎羽毛“快速”蛋白的信使核糖核酸的分离

Powell B C, Rogers G E

Nucleic Acids Res. 1979 Dec 20;7(8):2165-76. doi: 10.1093/nar/7.8.2165.

The messenger RNA coding for the "Fast" protein of embryonic chick feathers has been purified from the overwhelming relative amounts of keratin mRNA which are present in the developing feathers. The "Fast" protein mRNA represents about 4-8% of the total mRNA population of the feather. Despite differences between the size of the "Fast" proteins and the keratins the two mRNA species are very similar in molecular weight as judged by electrophoresis under denaturing conditions. However, by electrophoresis in 8 M urea gels at 55 degrees C, the "Fast" protein mRNA could be separated from keratin mRNA, presumably reflecting differences in messenger RNA secondary structure.

编码胚胎期雏鸡毛“快速”蛋白的信使核糖核酸已从发育羽毛中存在的大量相对含量的角蛋白信使核糖核酸中纯化出来。“快速”蛋白信使核糖核酸约占羽毛总信使核糖核酸群体的4% - 8%。尽管“快速”蛋白和角蛋白的大小存在差异，但在变性条件下通过电泳判断，这两种信使核糖核酸的分子量非常相似。然而，在55摄氏度下于8M尿素凝胶中进行电泳时，“快速”蛋白信使核糖核酸可与角蛋白信使核糖核酸分离，这大概反映了信使核糖核酸二级结构的差异。