从感染2型腺病毒的HeLa细胞核中分离出的病毒复制复合体的酶学特性。
Enzymatic properties of viral replication complexes isolated from adenovirus type 2-infected HeLa cell nuclei.
作者信息
Brison O, Kedinger C, Wilhelm J
出版信息
J Virol. 1977 Nov;24(2):423-35. doi: 10.1128/JVI.24.2.423-435.1977.
Abstract
When HeLa cell nuclei, isolated 17 h after infection with adenovirus type 2 (Ad2), were extracted with 200 mM ammonium sulfate, Ad2 nucleoprotein complexes were selectively released. These complexes contained a DNA polymerase activity that corresponded to DNA polymerase molecules actively engaged in Ad2 DNA replication. Under our high-salt (200 mM ammonium sulfate) incubation conditions, where no reinitiation occurred, full-length Ad2 DNA chains were synthesized by elongation of chains that had been initiated in vivo. This conclusion was further supported by density labeling experiments indicating that the in vitro DNA synthesis was semiconservative. Evidence is presented suggesting that at least part of the DNA polymerase molecules engaged in Ad2 DNA replication belong to the gamma class.
摘要
在用2型腺病毒（Ad2）感染17小时后分离得到的HeLa细胞核，用200 mM硫酸铵提取时，Ad2核蛋白复合物被选择性释放。这些复合物含有一种DNA聚合酶活性，该活性与积极参与Ad2 DNA复制的DNA聚合酶分子相对应。在我们的高盐（200 mM硫酸铵）孵育条件下，没有发生重新起始，全长Ad2 DNA链是通过体内已起始的链的延伸合成的。密度标记实验进一步支持了这一结论，表明体外DNA合成是半保留的。有证据表明，参与Ad2 DNA复制的DNA聚合酶分子至少部分属于γ类。
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