Greenwood M R, Hirsch J
J Lipid Res. 1974 Sep;15(5):474-83.
It has been shown by several investigators that adipocyte number is stable in mature human beings and several species of rodents. Although the number of new cells appearing in the adipose depot can be measured histometrically and by Coulter counting of osmium-fixed cells, such methods do not distinguish between "lipid filling" of preexistent adipocytes and synthesis of new adipocytes. The experiments reported here using in vivo injection of [(3)H]thymidine show that synthesis of new adipocytes in the Sprague-Dawley rat continues after birth and ceases before sexual maturity. Furthermore, during the second and third postnatal weeks, a "bed" of preadipocytes is synthesized. Preadipocytes may take as long as 30 days to appear as mature adipocytes.
几位研究者已表明，在成熟人类和几种啮齿动物中，脂肪细胞数量是稳定的。尽管可以通过组织计量学以及对锇固定细胞进行库尔特计数来测量脂肪库中出现的新细胞数量，但这些方法无法区分已存在脂肪细胞的“脂质填充”和新脂肪细胞的合成。此处报道的使用体内注射[³H]胸腺嘧啶核苷的实验表明，Sprague-Dawley大鼠出生后新脂肪细胞的合成仍在继续，并在性成熟前停止。此外，在出生后的第二和第三周，会合成一层前脂肪细胞“床”。前脂肪细胞可能需要长达30天才能发育为成熟脂肪细胞。
