Gaben-Cogneville A M, Swierczewski E
Lipids. 1979 Jul;14(7):669-75. doi: 10.1007/BF02533453.
[2-14C] Thymidine was injected into rats aged 3,5 and 10 days, and incorporation of the precursor into deoxyribonucleic acid (DNA) of the inguinal fat tissue was measured for short time periods. Using chromatographic procedures to measure the distribution of thymidine and its metabolites in the soluble fraction of the tissue, degradation of the precursor was found to be similar at all ages. The data indicate that thymidine was more rapidly utilized for DNA synthesis in 3-day-old rats than in older animals. When 14C-thymidine was injected in vivo and adipocytes and stromal cells were then separated from the inguinal tissue of 3-and 5-day-old rats, the incorporation into DNA was significant in both types of cells already 30 min after pulse labeling. Stromal cells took up twice as much of label as the adipocytes. Furthermore, real incorporation into DNA was found in the adipocytes when incubated in vitro in a culture medium supplemented with 14C-thymidine. The possibility is discussed that early in postnatal life adipocytes might synthesize DNA for further cell division.
将[2-¹⁴C]胸苷注射到3日龄、5日龄和10日龄的大鼠体内，并在短时间内测量前体掺入腹股沟脂肪组织脱氧核糖核酸（DNA）的情况。使用色谱程序测量胸苷及其代谢产物在组织可溶部分中的分布，发现所有年龄段前体的降解情况相似。数据表明，与年龄较大的动物相比，3日龄大鼠的胸苷用于DNA合成的速度更快。当将¹⁴C-胸苷进行体内注射，然后从3日龄和5日龄大鼠的腹股沟组织中分离出脂肪细胞和基质细胞时，脉冲标记30分钟后，两种类型的细胞中胸苷掺入DNA的情况均很显著。基质细胞摄取的标记物是脂肪细胞的两倍。此外，当在补充有¹⁴C-胸苷的培养基中进行体外培养时，在脂肪细胞中发现了真正掺入DNA的情况。文中讨论了出生后早期脂肪细胞可能合成DNA以进行进一步细胞分裂的可能性。
