Harada T, Yokobayashi K, Misaki A
Appl Microbiol. 1968 Oct;16(10):1439-44. doi: 10.1128/am.16.10.1439-1444.1968.
We have isolated a Pseudomonas sp. (strain SB15) which produces an isoamylase (EC 3.2.1.9). Highest yields of this enzyme were obtained when the bacterium was grown in shaken culture in a medium containing maltose, dextrin, starch, or isomaltose. Specific carbon and nitrogen sources were required for growth. The most satisfactory medium consisted of 2% maltose, 0.4% sodium glutamate, 0.3% diammonium hydrogen phosphate, and other inorganic salts. The optimal pH for enzyme production was 5 to 6. The enzyme is stable between pH 3 and 6 but is extremely labile above pH 7. It splits amylopectin completely by combined action with beta-amylase but does not attack pullulan.
我们分离出了一株假单胞菌（菌株SB15），它能产生一种异淀粉酶（EC 3.2.1.9）。当该细菌在含有麦芽糖、糊精、淀粉或异麦芽糖的培养基中振荡培养时，这种酶的产量最高。生长需要特定的碳源和氮源。最适宜的培养基由2%麦芽糖、0.4%谷氨酸钠、0.3%磷酸氢二铵和其他无机盐组成。产酶的最适pH为5至6。该酶在pH 3至6之间稳定，但在pH 7以上极不稳定。它通过与β-淀粉酶协同作用完全分解支链淀粉，但不作用于普鲁兰多糖。
