葡萄糖对大鼠胰岛中还原型/氧化型烟酰胺腺嘌呤二核苷酸磷酸细胞溶质比率的影响。
Effects of glucose on the cytosolic ration of reduced/oxidized nicotinamide-adenine dinucleotide phosphate in rat islets of Langerhans.
作者信息
Ashcroft S J, Christie M R
出版信息
Biochem J. 1979 Dec 15;184(3):697-700. doi: 10.1042/bj1840697.
Abstract
The maximal extractable activity of "malic" enzyme (EC 1.1.1.40) in rat islets of Langerhans was similar to that reported for liver. Thus "malic" enzyme may catalyse a near-equilibrium reaction in the cytosol of islets of Langerhans. Measurements of islet content of malate and pyruvate, the metabolite substrate and product of "malic" enzyme, were therefore used to calculate the cytosolic ration of [NADPH]/[NADP+]. This ratio was higher in islets incubated with 20 mM-glucose than in islets incubated with 2 mM-glucose.
摘要
大鼠胰岛中“苹果酸”酶（EC 1.1.1.40）的最大可提取活性与肝脏中报道的相似。因此，“苹果酸”酶可能在胰岛细胞溶胶中催化一个接近平衡的反应。因此，通过测量苹果酸和丙酮酸（“苹果酸”酶的代谢底物和产物）的胰岛含量，来计算细胞溶胶中[NADPH]/[NADP+]的比值。与在2 mM葡萄糖中孵育的胰岛相比，在20 mM葡萄糖中孵育的胰岛中该比值更高。
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