Ashcroft S J, Christie M R
Biochem J. 1979 Dec 15;184(3):697-700. doi: 10.1042/bj1840697.
The maximal extractable activity of "malic" enzyme (EC 1.1.1.40) in rat islets of Langerhans was similar to that reported for liver. Thus "malic" enzyme may catalyse a near-equilibrium reaction in the cytosol of islets of Langerhans. Measurements of islet content of malate and pyruvate, the metabolite substrate and product of "malic" enzyme, were therefore used to calculate the cytosolic ration of [NADPH]/[NADP+]. This ratio was higher in islets incubated with 20 mM-glucose than in islets incubated with 2 mM-glucose.
大鼠胰岛中“苹果酸”酶（EC 1.1.1.40）的最大可提取活性与肝脏中报道的相似。因此，“苹果酸”酶可能在胰岛细胞溶胶中催化一个接近平衡的反应。因此，通过测量苹果酸和丙酮酸（“苹果酸”酶的代谢底物和产物）的胰岛含量，来计算细胞溶胶中[NADPH]/[NADP+]的比值。与在2 mM葡萄糖中孵育的胰岛相比，在20 mM葡萄糖中孵育的胰岛中该比值更高。
