Cash R A, Music S I, Libonati J P, Schwartz A R, Hornick R B
Infect Immun. 1974 Oct;10(4):762-4. doi: 10.1128/iai.10.4.762-764.1974.
El Tor Ogawa C14-S5 and EW-6, two live vaccine candidate strains, were given to volunteers in varying doses with and without bicarbonate. Vibrios were found in the stool of one of 32 men given the vaccine strain, and only three men developed a significant titer rise (fourfold or greater) at 2 weeks of vibriocidal or antitoxic antibody. Five men who had previously received 10(9) organisms of the C14-S5 strain were challenged subsequently with virulent Ogawa 395 Vibrio cholerae. The rate of clinical infection in these men was no different than in unvaccinated controls. It was demonstrated that the live oral cholera vaccines did not remain viable in the intestine long enough to act antigenically.
两种活疫苗候选菌株，即埃尔托小川型C14 - S5和EW - 6，以不同剂量给予志愿者，有的加了碳酸氢盐，有的没加。在接种疫苗菌株的32名男性中，有1人的粪便中发现了弧菌，只有3人在2周时杀菌或抗毒素抗体出现显著滴度升高（四倍或更高）。5名之前接种过10⁹个C14 - S5菌株菌体的男性随后受到强毒小川型霍乱弧菌395的攻击。这些男性的临床感染率与未接种疫苗的对照组没有差异。结果表明，口服活霍乱疫苗在肠道内存活时间不够长，无法发挥抗原作用。
