豚鼠肝脏色氨酸吡咯酶。未检测到脱辅基酶活性，不存在皮质醇的激素诱导作用以及色氨酸可能的调节作用。
Guinea-pig liver tryptophan pyrrolase. Absence of detectable apoenzyme activity and of hormonal induction by cortisol and possible regulation by tryptophan.
作者信息
Badawy A A, Evans M
出版信息
Biochem J. 1974 Mar;138(3):445-51. doi: 10.1042/bj1380445.
Abstract
- When assayed in fresh homogenates, guinea-pig liver tryptophan pyrrolase exists only as holoenzyme. It does not respond to agents that activate or inhibit the rat liver enzyme in vitro. Only by aging (for 30min at 5 degrees C) does the guinea-pig enzyme develop a requirement for ascorbate. 2. The guinea-pig liver enzyme is activated by the administration of tryptophan but not cortisol, salicylate, ethanol or 5-aminolaevulinate. 3. The tryptophan enhancement of the guinea-pig liver pyrrolase activity is prevented by 0, 34 and 86% by pretreatment with actinomycin D, cycloheximide or allopurinol respectively. 4. The guinea-pig liver tryptophan pyrrolase is more sensitive to tryptophan administration than is the rat enzyme. On the other hand, the concentrations of tryptophan in sera and livers of guinea pigs are 45-52% less than those in rats. 5. It is suggested that tryptophan may regulate the activity of guinea-pig liver tryptophan pyrrolase by mobilizing a latent form of the enzyme whose primary function is the detoxication of its substrate.
摘要
- 在新鲜匀浆中进行测定时，豚鼠肝脏色氨酸吡咯酶仅以全酶形式存在。它对体外激活或抑制大鼠肝脏该酶的试剂无反应。仅通过老化（在5℃下30分钟），豚鼠酶才会产生对抗坏血酸的需求。2. 豚鼠肝脏酶可通过给予色氨酸而被激活，但不能被皮质醇、水杨酸盐、乙醇或5-氨基酮戊酸激活。3. 分别用放线菌素D、环己酰亚胺或别嘌呤醇预处理，可使豚鼠肝脏吡咯酶活性的色氨酸增强作用分别被抑制0%、34%和86%。4. 豚鼠肝脏色氨酸吡咯酶比大鼠酶对色氨酸给药更敏感。另一方面，豚鼠血清和肝脏中的色氨酸浓度比大鼠低45%-52%。5. 有人提出，色氨酸可能通过动员该酶的一种潜在形式来调节豚鼠肝脏色氨酸吡咯酶的活性，这种潜在形式的主要功能是对其底物进行解毒。
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