Salin M L, McCord J M
J Clin Invest. 1974 Oct;54(4):1005-9. doi: 10.1172/JCI107816.
Isolated human polymorphonuclear leukocytes were found to contain two distinct superoxide dismutases, electrophoretically identical to the superoxide dismutases found in other human tissues. One is inhibited by cyanide and is located in the cytosol. The other is not inhibited by cyanide and is mitochondrial. These findings disagree with reports by other laboratories, which did not find the cyanide-sensitive cytoplasmic enzyme. The superoxide dismutase-containing preparation of leukocytes released large amounts of superoxide into the medium when the cells were phagocytizing, suggesting that, if the cell population is homogeneous, superoxide production probably takes place on the outer surface of the cell membrane, in addition to inside the phagocytic vacuole.
已发现分离出的人多形核白细胞含有两种不同的超氧化物歧化酶，其电泳图谱与在其他人体组织中发现的超氧化物歧化酶相同。一种受氰化物抑制，位于细胞质中。另一种不受氰化物抑制，位于线粒体中。这些发现与其他实验室的报告不一致，其他实验室未发现对氰化物敏感的细胞质酶。当细胞进行吞噬作用时，含超氧化物歧化酶的白细胞制剂会向培养基中释放大量超氧化物，这表明，如果细胞群体是同质的，除了吞噬泡内部之外，超氧化物的产生可能还发生在细胞膜的外表面。
